Comparative analysis of expressed sequence tags from three castes and two life stages of the termite Reticulitermes flavipes

Table 3 In silico analysis and predicted protein function of 10 contigs with the highest R-value with no putative role assigned by a BLASTX search.

Library Bias	Contig #	Length (bp)	R¹	p-value²	Predicted Protein Function³
Soldier	910	1255	15	6.92^e-11	Antibacterial humoral response; Translation elongation
Alate	545	1346	382	6.39^e-128	Neurotransmitter secretion; Cholesterol absorption
Alate	142	1745	76	1.54^e-25	Cell adhesion; Hydrolase activity
Late Larval	366	478	97	5.45^e-70	Protein biosynthesis; Ligase activity
Late Larval	598	903	49	2.00^e-19	Regulation of transcription; Metabolism
Late Larval	516	393	39	2.04^e-16	Stress Response
Late Larval	533	474	31	7.12^e-13	Cell adhesion
Late Larval	582	1133	26	9.43^e-09	Spermatogenesis; Cell-cell adhesion
Late Larval	560	197	24	3.89^e-09	Ribosomal protein-nucleus import
Late Larval	596	747	14	1.27^e-07	Amino acid metabolism

¹ R-statistic was based on equations in Stekel et al. (2000) and compared relative abundance of each libraries prevalence in contiguous sequences from all available sequences.
² p-values were calculated to establish appropriate significance thresholds to confirm individual library abundance. p-value is in reference to significance of caste bias of singletons in contigs formed using a chi-squared test on singleton representation in each contig.
³ Putative protein functions were predicted using: http://dragon.bio.purdue.edu/pfp.

ISSN: 1471-2164