A complete DNA sequence map of the ovine Major Histocompatibility Complex

BMC Genomics

Table 1 Assembly of 26 BAC-clone based DNA sequences covering entire Ovine MHC region

BAC clone ID	GenBank Accession Number	Insert length (bp)	Average coverage^a	Single-base error probability^b	Reads number^c	High repeat sequence	Scaffolds^d	No. of Gaps inside^e	No. of Gaps outside
271H22	FJ985865	159959	7.93491	1.118 × 10^-4	2974	No	1	0	0
304C7	FJ985867	134586	8.0733	2.127 × 10^-4	2509	Yes	1	1	0
142M19	FJ985860	134479	8.11391	4.257 × 10^-4	2715	Yes	1	2	0
373D13	FJ985872	172485	8.15691	5.308 × 10^-4	3311	No	1	3	0
283N15	FJ985866	155021	7.165	0.268 × 10^-4	2472	No	1	0	0
222G18	FJ985862	167309	7.78757	0.790 × 10^-4	2783	No	1	0	0
55L9	FJ985854	145292	8.22565	0.195 × 10^-4	2941	No	1	0	0
197N2	FJ985876	90102	6.50404	0.488 × 10^-4	1438	No	1	2	0
429P24	FJ985873	198404	9.02502	3.497 × 10^-4	4009	No	1	2	0
225J15	FJ985863	139059	7.9057	68.30 × 10^-4	2335	Yes	1	1	0
453O11	FJ985874	143310	8.05201	0.802 × 10^-4	2473	No	1	2	0
63M17	FJ985856	129209	8.52801	37.780 × 10^-4	2394	No	1	1	0
163P3	FJ985861	165447	7.55517	1.049 × 10^-4	2833	No	1	0	0
119K1	FJ985858	156603	7.75008	0.309 × 10^-4	2665	No	1	2	0
349I12	FJ985871	149708	8.4984	7.600 × 10^-4	2994	No	1	2	0
345B17	FJ985869	134643	7.69046	10.58 × 10^-4	2736	No	2	0	1
68G10	FJ985857	165531	8.14164	6.535 × 10^-4	3681	Yes	1	1	0
346G21	FJ985870	138311	8.67944	9.059 × 10^-4	2807	No	1	1	0
44I10	FJ985853	134434	8.32584	16.47 × 10^-4	2705	No	1	2	0
282P19	FJ985875	174317	7.27217	0.490 × 10^-4	2989	No	2	1	1
239C1	FJ985864	142287	7.9438	0.736 × 10^-4	2940	No	1	2	0
141C4	FJ985859	160633	7.65743	0.624 × 10^-4	2992	No	1	1	0
374N21	FJ985877	83460	9.112	3.860 × 10^-4	3648	No	2	1	1
21H3	FJ985852	119055	8.13723	0.380 × 10^-4	2157	No	1	1	0
304D17	FJ985868	140735	8.14013	4.291 × 10^-4	2599	No	1	1	0
58G13	FJ985855	135958	8.827674	2.187 × 10^-4	3845	No	2	4	0

^a Defined as a ratio between total number of base pairs sequenced and total number of base pairs of the inserts in a given BAC clone.
^b Error probability of a particular base call, corresponding to a quality value as determined by the equation:
Q = -10log₁₀(P_e), where P_e is the error probability.
^c The total number of shotgun DNA sequencing reactions performed for a given BAC clone.
^d In genomic mapping, a series of contigs that are in the right order but not necessarily connected in one continuous stretch of sequence.
^e The number of regions where the exact nucleotide base (G, A, T, or C) could not determined, represented by a strips of "N" in a given BAC clone.

ISSN: 1471-2164