Figure 1

AMC region from third instar larvae observed by optical microscopy. (A) Bright-field view of the larval AMC region (dorsal view, anterior down), the hooks appear in dark. Cells that constitute the AMC are located on either side of the hooks. (B) 3D reconstruction of AMC (TO +DO), labelled with Pros (red) and Elav (green) that labels neuronal cells. (B'1-3) Zoomed view of a confocal section of the framed region in B showing respectively the Pros (B'1), Pros/Elav (B'2) and Elav (B'3) staining; Anti-Prospero labels two types of Pros expressing cells (Pros+): large (arrowheads in B'1) and small cells (arrows in B'1). Some of the small Pros+ cells express Elav (B'2). Scale bars represent 10 μm.