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Figure 2 | BMC Genomics

Figure 2

From: Gill transcriptome response to changes in environmental calcium in the green spotted puffer fish

Figure 2

Results from K -means clustering of differentially expressed SuperSAGE tags according to their expression patterns. Pattern of expression of all tags for each cluster (mean ± S.E.M of expression, normalized by making the sum of tag counts for all the analyzed libraries equal to 100%), (A) for the LowCa analysis (effects of transfer to 0.01 mM Ca2+ at 2 or 12 h), (B) for the 12 h analysis, describing tag expression at 12 h exposure to LowCa (0.01 mM Ca2+), control (2.9 mM Ca2+) or HighCa (10 mM Ca2+) water. The number of tags grouped in each cluster is shown in each graph. C- Bubble graph showing the relation between clusters of the two analyses. Bubble area is proportional to the number of tags in common by each pair of cluster number (LowCa and 12 h analysis). Tags falling in clusters numbered "zero" were those not analyzed (due to failure of inclusion criteria, see methods) for a given analysis (LowCa or 12 h). Solid line - control; dashed line - LowCa.

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