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Table 1 Overview of the results comparing use of oligo(dT) and multi-targeted primers (MTPs) for reverse transcription.

From: Multi-targeted priming for genome-wide gene expression assays

  Saccharomyces cerevisiae Neurospora crassa
Feature Nitrogen depletion Protoperithecial developmenr
  Oligo (dT) MTP Common Oligo (dT) MTP Common
Number of well measured genes1 on the array 4620 6042 4573 469   416
Number of genes significantly differentially expressed (P ≤ 0.05) 2155 3577 1839 172 406 2021
Number of up-regulated genes (P ≤ 0.05) 1080 1855 925 66 232 31
Number of down-regulated genes (P ≤ 0.05) 1075 1722 912 106 174 61
Highest gene expression ratio 49.15 32.6   24.26 10.74  
Average gene expression ratio 2.05 1.77   1.96 1.43  
GEL502 1.51 1.27   1.91 2.08  
Number of genes with gene expression ratio greater or equal to GEL50 (P ≤ 0.05) 1237 2617 1166 102 141 52
  1. 1 well measured genes recorded after data normalization (foreground fluorescence signals were at least three standard deviations of the distribution of intensities of the background pixels for that gene).
  2. 2 GEL50 - gene expression level at which there is a 50% empirical probability of a significant call.