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Table 2 Comparison of oligo(dT) and MTP priming in terms of number of genes significantly differentially expressed in specific biological processes and metabolic pathways with the highest number of genes significantly differentially expressed (P ≤ 0.05) in S. cerevisiae and N. crassa.

From: Multi-targeted priming for genome-wide gene expression assays

Process/pathway* Oligo(dT) MTP Additional genes identified by MTP Improvement (%)
Saccharomyces cerevisiae, nitrogen depletion
Carbohydrate Metabolism 136 208 72 53
Amino Acid Metabolism 114 181 67 59
Lipid Metabolism 55 89 34 62
Metabolism of Cofactors and Vitamins 43 67 24 56
Energy Metabolism 37 62 25 68
Glycan Biosynthesis and Metabolism 28 58 30 107
Xenobiotics Biodegradation and Metabolism 28 52 24 86
Nucleotide Metabolism 31 49 18 58
Signalling 19 39 20 105
Metabolism of Other Amino Acids 20 36 16 80
Cell cycle 20 32 12 60
Biosynthesis of Secondary Metabolites 9 15 6 67
Folding, Sorting and Degradation 7 12 5 71
Replication and repair 8 8 0 0
Translation 2 2 0 0
Transcription 1 1 0 0
Neurospora crassa, protoperithecial development
Carbohydrate metabolism 23 30 7 30
Translation 25 29 4 16
Amino acid metabolism 14 26 12 86
Lipid metabolism 8 19 11 138
Xenobiotics biodegradation and metabolism 5 17 12 240
Metabolism of other amino acids 3 11 8 267
Metabolism of cofactors and vitamins 3 10 7 233
Energy metabolism 9 8 -1 -11
Glycan biosynthesis and metabolism 0 5 5 **
Nucleotide metabolism 1 4 3 300
Biosynthesis of secondary metabolites 2 3 1 50
Signalling 1 1 0 0
Protein fate 0 1 1 **
Transcription 0 1 1 **
  1. * The pathways and processes were identified using Kyoto Encyclopedia of Genes and Genomes (KEGG). For full list of S. cerevisiae pathways, see Additional File 3.
  2. ** biological process not identified by oligo(dT) primers as including any genes significantly differentially expressed by N. crassa protoperithecia.