Table 2 Comparison of oligo(dT) and MTP priming in terms of number of genes significantly differentially expressed in specific biological processes and metabolic pathways with the highest number of genes significantly differentially expressed (P ≤ 0.05) in S. cerevisiae and N. crassa.
From: Multi-targeted priming for genome-wide gene expression assays
Process/pathway* | Oligo(dT) | MTP | Additional genes identified by MTP | Improvement (%) |
|---|---|---|---|---|
Saccharomyces cerevisiae, nitrogen depletion | ||||
Carbohydrate Metabolism | 136 | 208 | 72 | 53 |
Amino Acid Metabolism | 114 | 181 | 67 | 59 |
Lipid Metabolism | 55 | 89 | 34 | 62 |
Metabolism of Cofactors and Vitamins | 43 | 67 | 24 | 56 |
Energy Metabolism | 37 | 62 | 25 | 68 |
Glycan Biosynthesis and Metabolism | 28 | 58 | 30 | 107 |
Xenobiotics Biodegradation and Metabolism | 28 | 52 | 24 | 86 |
Nucleotide Metabolism | 31 | 49 | 18 | 58 |
Signalling | 19 | 39 | 20 | 105 |
Metabolism of Other Amino Acids | 20 | 36 | 16 | 80 |
Cell cycle | 20 | 32 | 12 | 60 |
Biosynthesis of Secondary Metabolites | 9 | 15 | 6 | 67 |
Folding, Sorting and Degradation | 7 | 12 | 5 | 71 |
Replication and repair | 8 | 8 | 0 | 0 |
Translation | 2 | 2 | 0 | 0 |
Transcription | 1 | 1 | 0 | 0 |
Neurospora crassa, protoperithecial development | ||||
Carbohydrate metabolism | 23 | 30 | 7 | 30 |
Translation | 25 | 29 | 4 | 16 |
Amino acid metabolism | 14 | 26 | 12 | 86 |
Lipid metabolism | 8 | 19 | 11 | 138 |
Xenobiotics biodegradation and metabolism | 5 | 17 | 12 | 240 |
Metabolism of other amino acids | 3 | 11 | 8 | 267 |
Metabolism of cofactors and vitamins | 3 | 10 | 7 | 233 |
Energy metabolism | 9 | 8 | -1 | -11 |
Glycan biosynthesis and metabolism | 0 | 5 | 5 | ** |
Nucleotide metabolism | 1 | 4 | 3 | 300 |
Biosynthesis of secondary metabolites | 2 | 3 | 1 | 50 |
Signalling | 1 | 1 | 0 | 0 |
Protein fate | 0 | 1 | 1 | ** |
Transcription | 0 | 1 | 1 | ** |