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Table 5 Validation of microarray and RNA sequencing of yeast grown in nitrogen poor (MM) and nitrogen rich (SC) conditions using Real Time RT PCR.

From: Multi-targeted priming for genome-wide gene expression assays

  Microarray RNA sequencing RT PCR
Gene ID MTP oligo(dT) MTP oligo(dT) MTP Oligo(dT)
  Ratio MM/SC p value Ratio MM/SC p value Ratio MM/SC p value Ratio MM/SC p value Ratio MM/SC Ratio MM/SC
YDL244W 1.98 0.00    3.16 0.11    28.94 39.62
YGR225W 1.72 0.00    7.65 0.00    4.08 3.13
YGR249W 1.66 0.01    5.80 0.00    4.79 8.20
YMR025W 1.51 0.00    3.96 0.15    2.10 1.57
YNR072W -1.24 0.05    -6.32 0.00    -2.34 -1.36
YFR034C -1.42 0.02 -1.25 0.10 -9.08 0.00 -6.41 0.08 1.30 1.24
YKR053C -5.37 0.00 -1.88 0.14 -2.16 0.00 1.15 0.51 -2.10 -2.05
YLR126C 1.54 0.00 1.36 0.11 5.54 0.05 1.40 0.42 1.58 1.95
YMR003W 1.25 0.01 1.11 0.26 7.91 0.01 2.65 0.08 1.03 1.95
YOR252W 1.29 0.01 1.03 0.46 6.33 0.00 1.54 0.10 1.99 1.54