Figure 2

Expression of nuclear mt genes with hypo T-DMRs. (A) Boxplots show the expression ratio (log₂ ratio) of the nuclear mt genes. Tissues used for comparisons are shown under the plots. "All" indicates that the plot shows the expression ratio of entire nuclear mt genes. "U" and "D" indicate that the plot shows the expression ratio of nuclear mt genes with hypo T-DMRs in upstream regions and downstream regions, respectively. The number of probe sets representing the expression levels of corresponding genes are displayed under the plot. P-values obtained from the Wilcoxon test are indicated on the top of the plot. (B) Genes which were relatively highly expressed in the tissues where downstream T-DMRs were hypomethylated, according to gene expression array data. Relative gene expression was confirmed using real-time PCR and was normalized to the expression of ß-actin (Actb). Bar graphs show the results of real-time PCR. "ND" indicates that the expression was not detected under the experimental condition used. Experiments were performed in biological duplicates, and all amplification were performed in triplicates. Error bars show standard deviations. For the Otc and Acsl6 genes, methylation levels of downstream hypo T-DMRs were examined by COBRA, and the electrophoresis images obtained by MultiNA microchip electrophoresis system are shown.