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Table 1 Results of Methylation Specific PCR analysis for five C. gigas genes

From: DNA methylation patterns provide insight into epigenetic regulation in the Pacific oyster (Crassostrea gigas)

Accession # Best blast hit [Organism] Undigested HpaII MspI Number of restriction sites CpG o/e
EW778441 heat shock protein 70
[Crassostrea gigas]
+ + _ 2 0.57
EW777519 heat shock protein 25
[Danio rerio]
+ _ _ 3 0.81
EW778166 cytochrome P450
[Haliotis diversicolor]
+ _ _ 1 0.85
EW778608 macrophage expressed protein 1-like protein [Crassostrea gigas] + _ _ 6 1.08
EW778905 14-3-3 protein gamma (Protein kinase C inhibitor protein 1
[Bos taurus])
+ _ _ 2 0.92
  1. Results of methylation status of five genes associated with immune response by MSP. PCR was carried out on undigested, HpaII digested, and MspI digested DNA. Presence (+) or absence (-) of PCR product is indicated. Number of CCGG restriction sites in the indicated sequence and CpGo/e ratios are also provided.