DNA methylation patterns provide insight into epigenetic regulation in the Pacific oyster (Crassostrea gigas)

BMC Genomics

Table 1 Results of Methylation Specific PCR analysis for five C. gigas genes

Accession #	Best blast hit [Organism]	Undigested	HpaII	MspI	Number of restriction sites	CpG o/e
EW778441	heat shock protein 70 [Crassostrea gigas]	+	+	_	2	0.57
EW777519	heat shock protein 25 [Danio rerio]	+	_	_	3	0.81
EW778166	cytochrome P450 [Haliotis diversicolor]	+	_	_	1	0.85
EW778608	macrophage expressed protein 1-like protein [Crassostrea gigas]	+	_	_	6	1.08
EW778905	14-3-3 protein gamma (Protein kinase C inhibitor protein 1 [Bos taurus])	+	_	_	2	0.92

Results of methylation status of five genes associated with immune response by MSP. PCR was carried out on undigested, HpaII digested, and MspI digested DNA. Presence (+) or absence (-) of PCR product is indicated. Number of CCGG restriction sites in the indicated sequence and CpGo/e ratios are also provided.

ISSN: 1471-2164