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Table 1 Comparison of phylotype coverage and diversity estimation of the 16S rRNA gene libraries at the 3% dissimilarity from the pyrosequencing analysis

From: Molecular analysis of the diversity of vaginal microbiota associated with bacterial vaginosis

Group

Reads

OTUs 1

Good 2

ACE

95% C.I.

Chao

95% C.I.

Shannon

Evenness 3

Simpson

BV-Negative

156,132

1,584

1,584

0.996

2,857

(2,714, 3,000)

2,500

(2,375, 2,625)

2.6133

0.2185

0.3793

BV-Positive

90,227

2,455

0.990

4,009

(3,808, 4,209)

3,694

(3,509, 3,879)

3.7965

0.3327

0.1240

  1. 1The operational taxonomic units (OTUs) were defined with 3% dissimilarity level.
  2. 2The coverage percentage (Good), richness estimators (ACE and Chao1) and diversity indices (Shannon and Simpson) were calculated using Good's method (Good, 1953) and the MOTHUR program.
  3. 3The Shannon index of evenness was calculated with the formula E = H/ln(S), where H is the Shannon diversity index and S is the total number of sequences in that group.