Table 1 Summary of candidate genes identified from microarray analyses analyzed with in situ hybridization.
Affy Probe ID | Gene Namea | Gene Symbol | Corroborate Microarrayb | Expression Domainc | Expression in spt Embryosd | Expression Studies Previously Reported | Identified by Array | Fold-Change in Array |
|---|---|---|---|---|---|---|---|---|
Dr.16366.1.S1_at | BI430182 e | BI430182 f | Yes | IM | downregulated | No | 4/5s | -5.54 |
Dr.14123.1.A1_at | zgc:171560 | zgc:171560 f | Yes | ubiquitous | downregulated | No | 4/5s | -4.51 |
Dr.16573.3.S1_x_at | zgc:112524 | zgc:112524 f | Yes | ubiquitous | downregulated | No | 4/5s | -3.49 |
Dr.12443.1.A1_at | zgc:92345 | zgc:92345 f | Yes | ubiquitous | downregulated | No | 4/5s | -2.73 |
Dr.1085.2.A1_at | zgc:110288 | zgc:110288 f | Yes | ubiquitous | downregulated | No | 4/5s | -2.45 |
Dr.12055.1.S1_at | CCAAT/enhancer binding protein (C/EBP), alpha | cebpa f | Yes | IM, ALPM | downregulated | Yes | 4/5s | -16.67 |
Dr.8064.1.S1_at | draculin | drl | Yes | IM | downregulated | Yes | 4/5 s and 21s | -15.64 and -3.65 |
Dr.3338.1.S1_at | hydroxymethylb ilane synthase a | hmbsa f | Yes | IM, midline structures | downregulated | Yes | 4/5s | -4.56 |
Dr.515.1.A1_at | GTP binding protein 1 | gtpbp1 f | Yes | IM, midline structures | downregulated | Yes | 4/5s | -2.68 |
Dr.7908.1.S2_at | calmodulin 1a | calm1a f, g | Yes | IM, neuroectoderm | downregulated | Yes | 4/5s | -2.11 |
Dr.4151.1.S1_at | Janus kinase 2a | jak2a | Yes | ubiquitous | downregulated | Yes | 4/5s | -4.64 |
Dr.21814.1.S1_at | zgc:136864 | zgc:136864 | No - Category A | ubiquitous | same | No | 4/5s | -5.73 |
Dr.1118.1.A1_at | zgc:153587 | zgc:153587 | No - Category A | ubiquitous | same | No | 4/5s | -4.44 |
Dr.883.1.A1_at | DnaJ (Hsp40) homolog, subfamily C, member 21 | dnajc21 | No - Category A | ubiquitous | same | No | 4/5s | -2.52 |
Dr.14906.1.A1_at | zgc:153390 | zgc:153390 | No - Category A | ubiquitous | same | No | 4/5s | -2.21 |
Dr.16120.1.S1_at | prostaglandin E synthase 2-like | ptgesl | No - Category A | ubiquitous | same | Yes | 4/5s | -4.98 |
Dr.3023.1.S1_at | zinc finger, FYVE domain containing 21 | zfyve21 | No - Category A | ubiquitous | same | Yes | 4/5s | -4.47 |
Dr.8412.1.A1_at | DEAD (Asp-Glu-Ala-Asp) box polypeptide 3 | ddx3 | No - Category A | ubiquitous | same | Yes | 4/5s | -3.7 |
Dr.4269.1.A1_at | zgc:64161 | zgc:64161 | No - Category A | ubiquitous | same | Yes | 4/5s | -3.56 |
Dr.4468.1.S1_at | ribonuclease H2, subunit B | rnaseh2b | No - Category A | ubiquitous | same | Yes | 4/5s | -2.97 |
Dr.7212.1.S1_at | nedd8 activating enzyme E1 subunit 1 | nae1 | No - Category A | ubiquitous | same | Yes | 4/5s | -2.84 |
Dr.12643.1.A1_at | zgc:77744 | zgc:77744 | No - Category A | ubiquitous | same | Yes | 4/5s | -2.71 |
Dr.15269.1.S1_at | zgc:56033 | zgc:56033 | No - Category A | ubiquitous | same | Yes | 4/5s | -2.68 |
Dr.14026.1.A1_at | zgc:66110 | zgc:66110 | No - Category A | ubiquitous | same | Yes | 4/5s | -2.68 |
Dr.7237.1.S1_at | zgc:63962 | zgc:63962 | No - Category A | ubiquitous | same | Yes | 4/5s | -2.51 |
Dr.7966.1.S1_at | ATPase, H+ transporting, lysosomal, V1 subunit E isoform 1 | atp6v1e1 f | No - Category B | polster, neuroectoderm | downregulated | No | 4/5s | -2.54 |
Dr.1791.1.S1_at | pdgfa associated protein 1 | pdap1 f | No - Category B | polster | downregulated | Yes | 4/5s | -4.25 |
Dr.14426.1.S1_at | pin2/trf1-interacting protein 1 | pinx1 f | No - Category B | polster, neuroectoderm, PM | downregulated | Yes | 4/5s | -3.04 |
Dr.9715.3.S1_a_at | exosome component 4 | exosc4 f | No - Category B | PM | downregulated | Yes | 4/5s | -3 |
Dr.7799.1.A1_at | zgc:56136 | zgc:56136 f | No - Category B | neuroectoderm | downregulated | Yes | 4/5s | -2.73 |
Dr.18052.1.S1_at | zinc finger protein 593 | znf593 f | No - Category B | neuroectoderm, PM | downregulated | Yes | 4/5s | -2.46 |
Dr.893.2.S1_at | LOC798291 | LOC798291 | No - Category C | PM | same | No | 4/5s | -2.32 |
Dr.9423.1.S1_at | zgc:73329 | zgc:73329 | No - Category C | PM | same | Yes | 4/5s | -3.25 |
Dr.1663.1.A1_at | transcription factor 25 (basic helix-loop-helix) | tcf25 | No - Category C | polster | same | Yes | 4/5s | -2.76 |
Dr.8822.1.A1_at | zinc finger E-box binding homeobox 2a | zeb2a | No - Category C | neuroectoderm | same | Yes | 4/5s | -3.6 |
Dr.25212.1.A1_at | calsyntenin 1 | clstn1 | No - Category C | Kupffer's vesicle, tail bud | same | Yes | 4/5s | -3.28 |
Dr.12545.1.S1_at | si:dkey-177p2.6 | si:dkey-177p2.6 | No - Category C | neuroectoderm, PM | same | Yes | 4/5s | -2.87 |
Dr.19223.1.S2_at | aldolase c, fructose-bisphosphate | aldoc | No - Category C | neuroectoderm | same | Yes | 4/5s | -5.23 |
Dr.25679.1.S1_at | solute carrier family 1 (glial high affinity glutamate transporter), member 3a | slc1a3a | No - Category C | neuroectoderm | same | Yes | 4/5s | -3.05 |
Dr.1002.1.S1_at | LOC568423 | LOC568423 f | No - Category D | ubiquitous | upregulated | No | 4/5s | -2.34 |
Dr.25683.5.A1_at | sphingosine-1-phosphate receptor 1 | s1pr1 f | No - Category D | neuroectoderm, tailbud | upregulated | Yes | 4/5s | -2.76 |
Dr.8183.1.S1_at | homeo box A11a | hoxa11a f | No - Category D | tailbud | upregulated | Yes | 4/5s | -2.35 |
Dr.5206.1.S1_at | aldehyde dehydrogenase 1 family, member A2 | aldh1a2 | Yes | PM, eyes | downregulated | Yes | 21s | -6.16 |
Dr.1817.1.A1_at | regulator of calcineurin family member 3 | rcan3 f | Yes | PM | downregulated | Yes | 21s | -2.72 |
Dr.23348.1.A1_at | bone morphogenetic protein 3 | bmp3 f, g | Yes | PM, neuroectoderm | downregulated | Yes | 21s | -1.68 |
Dr.610.1.S1_at | heat shock protein 90-alpha 1 | hsp90a.1 f, g | Yes | PM | downregulated | Yes | 21s | -2.26 |
Dr.345.1.S1_at | unc-45 homolog B (C. elegans) | unc45b f, g | Yes | PM | downregulated | Yes | 21s | -2.39 |
Dr.26455.1.S1_at | fibroblast growth factor receptor-like 1a | fgfrl1a f, g | Yes | PM, eyes | downregulated | Yes | 21s | -1.73 |