Experimental evolution, genetic analysis and genome re-sequencing reveal the mutation conferring artemisinin resistance in an isogenic lineage of malaria parasites

Table 3 Unique mapping of reads to AS-WTSI reference sequence.

	number of bases mapped		% of genome sequence (WTSI) mapped
	in silico (unique)		experimental (unique)
			AS		AS-30CQ
			> = 5	> = 10	> = 5	> = 10
36 mer	18,574,867	98.63	97.78	95.88
41 mer	18,606,976	98.80			97.95	96.96

In silico analysis shows the number of bases and percentage of genome which can be uniquely mapped by sequence reads (equivalent to theoretical maximum). Experimental analysis shows the percentage of genome actually mapped by such reads, at different read-coverages (> = 5, > = 10) in AS-sens or AS-30CQ. AS-sens and AS-30CQ were analysed with 36 - and 41 base reads, respectively.

ISSN: 1471-2164