Figure 4

AS events without RefSeq Reports. The grey bars along the top of each diagram represent the RefSeq gene annotation. Traces in the upper region of each figure show average intensities of probesets representing exon regions for tumors (blue) and Cells (red). The lower insets show extensive annotatation provided by the H-InvDB. RefSeq annotation is shown in blue and ENSEMBL in orange (a) RefSeq annotates a single transcript for the FLOT1 gene while the H-InvDB reports annotation for 7 variants shown in the inset. None of the additional transcripts concur with the profile provided by the array. (b) The NES gene shows differential expression of 2 probesets in the 3' exon. RefSeq reports a single variant of this gene, H-InvDB reports 11 variants. The region on the 3' exon that shows higher expression in the tumors corresponds to AS events in the first and last transcripts (shown by arrows) reported by H-InvDB. (c) The C14orf149 gene appear to be differentially spliced between the two tissue types at exons 3,4 and part of 5 of. RefSeq details a single transcript for this gene, while H-Inv reports 4 alternatively spliced variants. None of the reported variants explain the differences between the two tissues profiles obtained from the exon array analysis.