Phylogenetic analysis of the three LIII subgroups. (A) A rooted tree shows the phylogenetic relatedness of the 9 LIII strains analyzed by CGH and 1 sequenced LIII strain HCC23. The tree was rooted by EGD-e and reconstructed based on the presence or absence of 3,560 HGs using the maximum-likelihood gene content method. Two branches with bootstrap values lower than 70% (1,000 replicates) are highlighted in red. (B) Neighbor-net split network shows the phylogenetic relatedness of 10 LIII strains. (C) A heat map based on PF values shows the distribution of 206 phylogenetically informative LI and LII core genes in 10 LIII strains.