Skip to main content
Figure 5 | BMC Genomics

Figure 5

From: Transcriptome and expression profiling analysis revealed changes of multiple signaling pathways involved in immunity in the large yellow croaker during Aeromonas hydrophila infection

Figure 5

The expression analysis of selected genes from the expression profile by relative quantitative real-time PCR. Total RNA was extracted from spleens of fish infected with A. hydrophila or injected with 0.9% NaCl. Real-time PCR was used to validate gene expression changes in the TLR pathway (A), cytokines (B), the MAPK signaling pathway (C), and the TCR signaling pathway (D). Increases and decreases in relative levels of transcripts with respect to the control β-actin gene are shown. For each gene, the black bar indicates the gene expression ratio of fish injected with 0.9% NaCl and is defined as 1; the grey bar indicates the expression ratio of fish infected with A. hydrophila, with associated standard error bars. Statistical significance of the relative expression ratio is indicated (*, P < 0.01).

Back to article page