Figure 5

Candidate master switch molecules and signaling pathways involved in LPS-induced inflammatory response of monocyte THP-1 cells. (A) Key node analysis was used to assess the possible signaling pathways or potential interactions in response to LPS stimulation only, at different time points. Pink circle: the putative master regulator/molecule; red box: the up-regulation (≥ 3-fold) of gene expression induced by LPS compared to vehicle treatment; green box: the down-regulation (≥ 3-fold) of gene expression induced by LPS compared to the vehicle treatment; gray circle: assigned molecule in the putative pathway; ↑: activation; ⊥: inhibition. (B) TRANSPATH analysis of common signaling denominators. Genes with the same expression mode in the "early non-response" and "early down-regulation" groups in both BF/S+L/Ep and cytopiloyne treatments were analyzed. Only 4 hierarchical levels of regulation were accepted.