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Table 1 AFLP primer combinations used, and the number and size of polymorphic fragments, and their segregation ratios.

From: Near-saturated and complete genetic linkage map of black spruce (Picea mariana)

Primer combinations

Size of fragments (bp)

Total number of polymorphic markers

Mendelian segregation

   

1:1

3:1

A-EAAC-MCCAC

54-340

52

31

21

A-EAAC-MCCACC

54-134

2

2

0

A-EAAC-MCCAG

65-502

17

17

0

A-EAAC-MCCATC

95-604

31

22

9

A-EAAG-MCCAG

80-236

18

11

7

A-EAAG-MCCATC

66-324

25

20

5

A-EACA-MCCAT

56-264

7

7

0

A-EACG-MCAT

52-198

22

14

8

A-EACG-MCCAG

40-245

14

8

6

A-EACG-MCTG

73-275

13

2

11

A-EACT-MCCAA

54-321

35

26

9

A-EACT-MCCTA

54-475

28

20

8

A-EAGC-MCTC

60-547

35

9

26

A-EAGC-MCTG

45-660

47

25

22

A-EACG-MCCAA

126-275

14

7

7

A-EACG-MCCTA

60-430

30

12

18

A-EACG-MCCGC

56-622

10

8

2

A-EACT-MCCAC

59-100

5

1

4

A-EACT-MCCAG

56-70

7

3

4

A-EACT-MCTA

74-327

32

19

13

A-EACT-MCAC

61-397

33

22

11

A-EACT-MCAT

80-262

27

15

12

A-EACG-MCCAT

66-148

8

4

4

A-EACG-MCCAC

67-327

11

4

7

A-EACG-MCTC

90-280

8

4

4

A-EACG-MCCAGC

78-210

12

11

1

A-EAAC-MCCTA

62-240

27

13

14

A-EAAC-MCCAT

69-414

23

13

10

A-EAAC-MCCAA

67-271

25

14

11

A-EAAC-MCCGA

66-194

11

6

5

A-EACA-MCAC

67-292

24

15

9

A-EACA-MCCACC

69-391

21

16

5

A-EACA-MCCGA

67-262

20

11

9

A-EACA-MCTA

60-261

33

26

7

A-EACA-MCCAC

67-178

9

7

2

A-EAAG-MCCAT

66-276

18

12

6

A-EAAG-MCCACC

65-121

9

5

4

A-EAAG-MCCAC

64-311

18

9

9

A-EAAG-MCCAA

69-249

11

7

4

A-EAAG-MCCTA

66-153

17

7

10

Total

 

809

485

324