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Table 1 AFLP primer combinations used, and the number and size of polymorphic fragments, and their segregation ratios.

From: Near-saturated and complete genetic linkage map of black spruce (Picea mariana)

Primer combinations Size of fragments (bp) Total number of polymorphic markers Mendelian segregation
    1:1 3:1
A-EAAC-MCCAC 54-340 52 31 21
A-EAAC-MCCACC 54-134 2 2 0
A-EAAC-MCCAG 65-502 17 17 0
A-EAAC-MCCATC 95-604 31 22 9
A-EAAG-MCCAG 80-236 18 11 7
A-EAAG-MCCATC 66-324 25 20 5
A-EACA-MCCAT 56-264 7 7 0
A-EACG-MCAT 52-198 22 14 8
A-EACG-MCCAG 40-245 14 8 6
A-EACG-MCTG 73-275 13 2 11
A-EACT-MCCAA 54-321 35 26 9
A-EACT-MCCTA 54-475 28 20 8
A-EAGC-MCTC 60-547 35 9 26
A-EAGC-MCTG 45-660 47 25 22
A-EACG-MCCAA 126-275 14 7 7
A-EACG-MCCTA 60-430 30 12 18
A-EACG-MCCGC 56-622 10 8 2
A-EACT-MCCAC 59-100 5 1 4
A-EACT-MCCAG 56-70 7 3 4
A-EACT-MCTA 74-327 32 19 13
A-EACT-MCAC 61-397 33 22 11
A-EACT-MCAT 80-262 27 15 12
A-EACG-MCCAT 66-148 8 4 4
A-EACG-MCCAC 67-327 11 4 7
A-EACG-MCTC 90-280 8 4 4
A-EACG-MCCAGC 78-210 12 11 1
A-EAAC-MCCTA 62-240 27 13 14
A-EAAC-MCCAT 69-414 23 13 10
A-EAAC-MCCAA 67-271 25 14 11
A-EAAC-MCCGA 66-194 11 6 5
A-EACA-MCAC 67-292 24 15 9
A-EACA-MCCACC 69-391 21 16 5
A-EACA-MCCGA 67-262 20 11 9
A-EACA-MCTA 60-261 33 26 7
A-EACA-MCCAC 67-178 9 7 2
A-EAAG-MCCAT 66-276 18 12 6
A-EAAG-MCCACC 65-121 9 5 4
A-EAAG-MCCAC 64-311 18 9 9
A-EAAG-MCCAA 69-249 11 7 4
A-EAAG-MCCTA 66-153 17 7 10
Total   809 485 324