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Table 1 Differential expression of proteins from fins by 2D-DIGE (Exp1, Exp2 and Exp3) and of their corresponding transcripts by hybridization to oligo microarrays

From: Zebrafish fin immune responses during high mortality infections with viral haemorrhagic septicemia rhabdovirus. A proteomic and transcriptomic approach

Spot n° Protein Name Accession Number ~MW ×10-3 ~pI fold Exp1 fold Exp2 fold Exp3 microarray
SIGNALING
47 hmgb1 42476233 16 7.0 2.4 *3.7 --- 1.01
26 gnb1 (G prot), βpolypep1 47087315 35 5.6 2.6 3.0 --- 1.03
APOPTOSIS
27 Annexin A1a 31419751 40 6.0 2.3 9.1 --- 1.01
39 Annexin 1a 27762256 44 6.2 2.5 --- --- N.F.
0 Annexin A5b 41107552 36 5.2 --- --- -2.0 -1.08
0 Annexin 5b 160773369 36 5.3 --- --- -1.9 N.F.
DETOXIFICATION
34 Glutathion S-Transferase 18858197 16 8.2 11.0 *2.7 --- 1.30
32 Glutathion S-Transferase 47086689 20 6.4 4.0 *2.1 --- 1.30
HAEMATOPOIESIS
10 Transferrin 51859259 70 6.9 16.1 *4.6 --- 2.40
11 Hemopexin 33991748 65 6.3 2.5 *3.2 --- 5.42
12 Hemopexin 33991748 65 6.5 3.5 --- --- 5.42
GLYCOLYSIS & ATP
24 Aldolase fructose-bisphosphate 37595414 44 8.3 5.7 8.2 *1.4 1.15
45 Triosephosphate isomerase 1b 47271422 18 7.1 2.1 *1.5 1.5 1.00
29 GAPDH 56718619 42 7.2 5.3 10.2 *1.8 1.12
31 GADPH 53733367 40 8.2 4.2 8.7 --- 1.12
4 GAPDH 53733367 85 5.6 -7.4 --- -*1.7 1.12
16 Alpha enolase 1 37681795 57 6.7 6.0 17.6 1.8 1.18
28 Ldhb Lactate dehydrogenase 28277619 35 6.8 3.3 --- *2.2 1.04
33 Carbonic anhydrase 18858379 22 7.8 10.4 --- --- -1.04
43 Malate dehydrogenase 47085883 40 7.8 2.1 *1.7 --- 1.05
3 ATP binding 51571925 85 5.5 -19.1 --- --- -2.22
0 ATP synthase 41152334 24 7.0 --- --- 1.5 1.05
46 Creatine kinase 55716037 15 6.5 -3.4 --- -*1.7 3.16
20 Creatine kinase 55716037 48 6.4 -3.9 --- -*2.0 3.16
5 Creatine kinase 55716037 90 5.9 -4.0 --- -*2.0 3.16
CYTOSKELETON & RELATED PROTEINS
42 Keratin 18 41351240 57 5.3 3.2 11.2 --- 1.08
38 Keratin type I 50370316 57 5.6 2.4 --- --- 1.09
13 Type II cytokeratin 18858425 59 5.1 3.2 1.0 --- 1.23
50 Tubulin, gamma assoc protein 2 41056243 85 6.0 2.4 --- --- -1.01
30 Skeletal alpha-actin (S. aurata) 6653228 38 7.9 -2.7 -2.8 --- -1.53
21 Alpha-tropomyosin 18859505 44 6.6 --- 3.4 7.2 1.56
0 Alpha-tropomyosin 55962544 36 5.0 --- --- 11.7 1.56
15 Transgelin 37681953 49 5.2 -7.4 --- --- 1.49
22 Kinesin-like protein 50055013 50 6.7 -2.7 --- --- -2.5
41 Myosin VIa 10116291 114 5.9 2.0 *5.1 7.7 1.05
0 Myosin, light chain 2 18859049 22 5.0 --- --- 7.3 1.30
19 CapG 29612467 48 5.3 2.8 *7.6 --- -1.08
0 Actin capping protein 41053959 34 5.7 --- --- -1.6 1.09
6 Scinderin 42542770 80 6.4 4.1 --- --- -1.14
7 Scinderin 42542770 80 6.5 3.8 --- --- -1.14
17 Cyclase-associated protein-1 37725381 58 8.3 5.0 --- --- 1.66
35 Dynamin 1-like 41055508 5 5.3 3.0 --- --- 1.01
48 Semaphorin 3Gb 57790316 7 6.4 3.0 --- --- 1.09
14 CkII protein 39645432 65 5.3 2.9 --- --- 1.13
0 fgf20 fin regeneration 51571925 26 6.2 --- --- 1.8 1.26
0 fgf20 fin regeneration 51571925 26 6.9 --- --- 2.2 1.26
0 fgf20 fin regeneration 51571925 26 7.0 --- --- 2.3 1.26
0 fgf20 fin regeneration 51571925 26 7.2 --- --- 1.8 1.26
  1. Exp1, experiment 1. Exp2, experiment 2. Exp3, experiment 3. Spot n°, number of spot selected in the 2 D gel of Exp1 (Figure 2) for mass spectrophotometry (MS) identification. 0, numbers of spots selected in 2 D gels of exp 2 or 3 (not shown). Gel spots were digested with trypsin and their peptides identified by MS. The corresponding detected proteins with a 100% of probability score of identification are shown grouped by their function. *, spots hypothetically identified by their apparent molecular weight (MW) and isoelectric point (IP) in their respective 2 D gels. ---, spots too faint to be quantified. ~MW and ~PI, apparent molecular weight (MW) and isoelectric points (IP) in the 2 D gel. Accession number of the identified protein sequences in the protein bank. Fold, calculated by the DeCyder-differential in-gel analysis software by the formula, volume of the spot in the VHSV-infected fin/volume of the spot in the non-infected fin. The spots on Figure 2 which have no corresponding description on Table 2 could not be identified by MS. Microarray differential expression data was obtained as explained in the legend of Table 3. Because most of the protein genes appeared several times in the microarray, their corresponding folds were calculated as the means for each of their data (n = 3-10). NF, not found annotated in the microarray.