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Table 2 Differential expression of transcripts from fins and organs by Q-PCR arrays.

From: Zebrafish fin immune responses during high mortality infections with viral haemorrhagic septicemia rhabdovirus. A proteomic and transcriptomic approach

   

fold

accession number

short name

long name transcripts

Fins

Organs

complement components

NM_001024435

c9

complement component 9

15.33

2.71

NM_131243

c3b

complement component c3b

9.24

-1.30

NM_200863

c8g

complement component 8. gamma polypeptide

3.69

1.63

immunoglobulin-related proteins

NM_001034182

sid4

secreted immunoglobulin domain 4

35.99

1.09

cluster differentiation antigens

NM_001002363

cd36

CD36 antigen

7.68

1.26

NM_212619

cd9

CD9 antigen (p24)

2.09

1.25

interleukins

NM_001020792

il22

interleukin 22

3.59

-1.32

NM_001020789

il17d

interleukin 17d

2.20

1.04

major hystocompatibility complex

NM_131471

mhc1uba

major histocompatibility complex class I UBA gene

3.01

1.41

guanine nucleotide binding proteins

NM_213224

gnl2

guanine nucleotide binding protein-like 2 (nucleolar)

2.72

-1.09

NM_001002397

gng7

guanine nucleotide binding protein gamma 7

2.02

1.21

Various

NM_152980

mst1

macrophage stimulating 1 hepatocyte growth factor

25.02

-1.36

NM_131607

tradd

tnfrsf1a-associated via death domain

2.83

1.07

NM_001110278

acvr2a

activin receptor IIa

2.49

-1.01

NM_001039637

foxp1b

forkhead box P1b

2.37

1.09

NM_200154

sla/lpl

soluble liver antigen/liver pancreas antigen. like

2.28

2.10

NM_131000

alcam

activated leukocyte cell adhesion molecule

2.25

-1.29

NM_001040353

crfb12

cytokine receptor family member B12

1.68

-3.33

  1. For each experiment, 10 zebrafish were infected with 2 × 106 ffu of VHSV/ml at 14°C while other 10 zebrafish remained non-infected. RNA was extracted from the fins or organs 2-days after VHSV or mock infection, converted to cDNA and used for the AmpliTaq reaction in 384 well plates containing 2 × 186 selected immune-related zebrafish TaqMan Assays (Applied Biosystems). In each plate, 2 × 3 assays of the rplp0 gene were used for normalization. The mean and their corresponding p (t Student one tail) were then calculated from 5 experiments. The transcripts from either fins and/or organs with >2-fold and p < 0.05 changes were first tabulated and then the rest of the table was completed with their corresponding calculated fold values in organs and/or fins, respectively. Fold, expression level in VHSV-infected tissue/expression level in non-infected tissue. +, increased. -, decreased. ~, similar. Figure 3 shows the representation of means and standard errors (SE) of all the genes.