The CAZyome of Phytophthora spp.: A comprehensive analysis of the gene complement coding for carbohydrate-active enzymes in species of the genus Phytophthora

Table 1 Gene-specific primers*

Gene	Forward primer sequence	Reverse primer sequence	T_m	Amplicon Size
PITG_08590	CATCTGTCTGGGCAGCGTGT	TTCATCGAGACCCACGAACG	65	190
PITG_08421	ATGGCACAACAGCAATGGGA	GCCCAGCAATACCCAGCTTC	65	190
PITG_08910	TAACGGCAACGAGAATGCGA	CAGTGCTGCTGGATTCGTCC	65	188
PITG_18907	CGTGGTTCGAGTCTTGCGAC	AGCTCGCTGTTCTGCCACAC	64	184
PITG_08914	CGTCAGCTCTACGCACGATCA	TGCTTCTGCCACACAACGC	65	194
PITG_02545	AGGCTCGTGTCTTCGGTTCC	GCCCCTGCTCCTCTGTTGTT	64	190
PITG_08863	CGGCAACAAGAACGCTACCA	TTGTCGTCCCAAGGTGTCCA	64	189
PITG_08912	TCAGCCACACTTGCTACGGC	TGTTCTTGGTAGAGGCCGGG	65	197
PITG_08911	TCTTCGGCAAACTCGCTCAA	CCTTGCGGGTGGATAACGTC	64	199
PITG_07333	GAGCTGGCAAATCAGACGCA	TTCGGGTCGATGTTTTCGTG	65	185
PITG_10850	TCTGTGAAGGCAGGGACGTG	ATAACTGCACCCAGCACGCA	65	182
PITG_02504	AACGGGCAGCAGACCTATCG	GGACACATCAGCATCGGGTG	65	185
PITG_14190	TCCTGTTCAAACTCGTGCTG	TCTGGGAACTTGGGGTGTAG	60	160
PITG_07334	TGCGCTACATGTTCCTTGAC	ATGTGCTCCATCCCATTAGC	60	160
PITG_11976	GCTAAACGGAGATGGACTGC	GCGAAAAAGTAACCGTGGAG	60	111
PITG_03543	TGATCGCAGACAGCTACGTC	GAAGCGCATAGAAGTACCGC	60	174

*Carbohydrate esterase gene sequences from Phytophthora infestans were used to design gene-specific primers for RT-PCR and qPCR. Gene names correspond to the descriptors given in assembly 1.0 of the P. infestans genome.

ISSN: 1471-2164