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Table 3 cDNA libraries from four G. clavigera isolate-treatment combinations and numbers of high quality ESTs derived from each library.

From: Gene discovery for the bark beetle-vectored fungal tree pathogen Grosmannia clavigera

Library Iso-lates Media/treatment combinations Norma-lized Primary titre (cfu/ml) ESTs sequenced ESTs HQ (%)
OCL01 1 W:S:ON:IN:OO No 2.1 × 106 6,144 5,272 (86)
OCL02 1 W:S:ON:IN:OO Yes 2.1 × 105 15,360 14,075 (92)
OCL03 1 ON+LPPE No 4.0 × 105 6,144 5,618 (91)
OCL04 1 ON+LPPE Yes 2.5 × 105 9,216 7,794 (85)
OCL05 2-8 W:S:ON:IN:OO:ON+LPPE No 2.0 × 106 3,072 2,916 (95)
OCL06 2-8 W:S:ON:IN:OO:ON+LPPE Yes 9.0 × 104 3,072 2,819 (92)
OCL08 1 Sp No 1.3 × 106 6,912 5,794 (84)
  1. For the libraries OCL01-OCL06, mycelial cultures from each of the eight isolates were grown on five different media, and for one medium the mycelia were treated with LPPE (W = wood, S = starch, ON = organic nitrogen, IN = inorganic nitrogen, OO = olive oil, LPPE = treatment with lodgepole pine methanol extract). After extracting total RNA separately from each of the 48 isolate/media/LPPE-treatment variants and from the spore sample (Sp), equal amounts of total RNA were pooled to obtain four isolate/treatment combinations. From these pooled samples we purified poly(A+) mRNA and generated cDNA. All three non-spore cDNA samples were divided into two fractions, one of which was normalized. The resulting seven cDNA samples were used for library construction. HQ = high quality.