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Table 3 cDNA libraries from four G. clavigera isolate-treatment combinations and numbers of high quality ESTs derived from each library.

From: Gene discovery for the bark beetle-vectored fungal tree pathogen Grosmannia clavigera

Library

Iso-lates

Media/treatment combinations

Norma-lized

Primary titre (cfu/ml)

ESTs sequenced

ESTs HQ (%)

OCL01

1

W:S:ON:IN:OO

No

2.1 × 106

6,144

5,272 (86)

OCL02

1

W:S:ON:IN:OO

Yes

2.1 × 105

15,360

14,075 (92)

OCL03

1

ON+LPPE

No

4.0 × 105

6,144

5,618 (91)

OCL04

1

ON+LPPE

Yes

2.5 × 105

9,216

7,794 (85)

OCL05

2-8

W:S:ON:IN:OO:ON+LPPE

No

2.0 × 106

3,072

2,916 (95)

OCL06

2-8

W:S:ON:IN:OO:ON+LPPE

Yes

9.0 × 104

3,072

2,819 (92)

OCL08

1

Sp

No

1.3 × 106

6,912

5,794 (84)

  1. For the libraries OCL01-OCL06, mycelial cultures from each of the eight isolates were grown on five different media, and for one medium the mycelia were treated with LPPE (W = wood, S = starch, ON = organic nitrogen, IN = inorganic nitrogen, OO = olive oil, LPPE = treatment with lodgepole pine methanol extract). After extracting total RNA separately from each of the 48 isolate/media/LPPE-treatment variants and from the spore sample (Sp), equal amounts of total RNA were pooled to obtain four isolate/treatment combinations. From these pooled samples we purified poly(A+) mRNA and generated cDNA. All three non-spore cDNA samples were divided into two fractions, one of which was normalized. The resulting seven cDNA samples were used for library construction. HQ = high quality.
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BMC Genomics

ISSN: 1471-2164

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