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Table 7 Consistency in detection of differential expression between quantities of starting RNA

From: The cost of reducing starting RNA quantity for Illumina BeadArrays: A bead-level dilution experiment

 

reference experiment

test experiment

250 ng

100 ng

50 ng

10 ng

0.62/0.04

0.67/0.04

0.69/0.05

50 ng

0.83/0.07

0.88/0.08

 

100 ng

0.86/0.06

  
  1. For the numbers of bead-types for which differential expression between UHRR and Brain was detected we report "X/Y" where X is the proportion of bead-types reported for the reference experiment also reported for the test experiment (e.g. a measure sensitivity), and Y is the proportion of bead-types reported by the test experiment that were not reported by the reference experiment (e.g. FDR). So for example, taking 250 ng as a gold-standard, for this detection measure the 100 ng experiment has 86% sensitivity and an FDR of 0.06.