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Table 1 Cq values obtained by qPCR using RNA and AA-aRNA

From: An optimized protocol for microarray validation by quantitative PCR using amplified amino allyl labeled RNA

Gene

RNA

AA-aRNA

VEGFB

20.1

27.7

MMP9

27.3

ND

TFRC

18.4

24.6

HAMP

23.3

23.6

GAPDH

14.5

16.5

Mean ± SD

20.7 ± 4.9

25.6 ± 7.0 *

  1. qPCR was performed on universal reference RNA and AA-aRNA using primer pairs specific for the indicated genes. ND: not detectable. * P = 0.02 vs RNA (one way repeated measures ANOVA).