Table 3 List of primers used in this study
Gene | cDNA length (bp) | Genbank accession number | Forward primer | Reverse primer | Distance from 3' end (bp) |
|---|---|---|---|---|---|
ANXA1 | 1399 | NM_000700 | GGAACGCTTTGCTTTCTCTTG | TTCTGGTGGTAAGGATGGTATTG | 795 |
ACTB | 1852 | NM_001101 | AGAAAATCTGGCACCACACC | GGGGTGTTGAAGGTCTCAAA | 1520 |
ELANE | 938 | NM_001972 | CGGGCTAATCCACGGAATTG | TTGTCCTCGGAGCGTTGG | 273 |
GAPDH | 1310 | NM_002046 | CAGCCTCAAGATCATCAGCA | TGTGGTCATGAGTCCTTCCA | 782 |
HAMP | 430 | NM_021175 | AGTGGCTCTGTTTTCC | GAAGTGGGTGTCTCG | 292 |
HIF1A | 4082 | NM_001530 | AGAAGGTATGTGGCATTTATTTGG | CAGGGTAGGCAGAACATTTAGG | 516 |
CGTGTTATCTGTCGCTTTGAGTC | TTTCGCTTTCTCTGAGCATTCTG | 1611 | |||
TTGGCAGCAACGACACAG | GCAGGGTCAGCACTACTTC | 2440 | |||
AGCCGAGGAAGAACTATGAAC | ACTGAGGTTGGTTACTGTTGG | 3149 | |||
CCTGACAAGCCACCTGAG | TCGTGAGACTAGAGAGAAGC | 3892 | |||
LY96 | 619 | NM_015364 | TGCCGAGGATCTGATGAC | ATTAGGTTGGTGTAGGATGAC | 245 |
MMP9 | 2387 | NM_004994 | AACTACGACACCGACGAC | CAGGCGGAGTAGGATTGG | 1587 |
STAT3 | 4900 | NM_003150 | GCTGGCTGACTGGAAGAG | AGTTGAGATTCTGCTAATGACG | 3943 |
TFRC | 5241 | NM_003234 | ATTGAACCTGGACTATGAGAG | GGAAGTAGCACGGAAGAAG | 3140 |
TNF-α-1 | 1669 | NM_000594 | AGTGACAAGCCTGTAGCC | GGACCTGGGAGTAGATGAG | 1243 |
TNF-α-2 | 1669 | NM_000594 | AAACAATGCTGATTTGGTGAC | GGCGATTACAGACACAACTCC | 119 |
VEGFB | 1172 | NM_003377 | CTGTGGTGGCTGCTG | ACTGGCTGTGTTCTTCC | 901 |