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Figure 2 | BMC Genomics

Figure 2

From: Genome analysis and comparative genomics of a Giardia intestinalis assemblage E isolate

Figure 2

Evolutionary analyses of Giardia genes compared between assemblage A and E. The evolutionary rate of Giardia genes between P15 and WB was examined using estimates of dN/dS. Genes were grouped into gene ontology (GO) categories. The boundaries of each box plot represents the lower quartile (Q1 = 25%) and the upper quartile (Q3 = 75%) and the median value is displayed by the black line. The box width is proportional to the number of genes in the group. Statistical outliers are represented by circles. (A) The distribution of dN/dS estimates for genes with ortholog relationships between P15 and WB. The vertical axis shows fraction of the genes with a certain dN/dS ratio (horizontal axis). The curve is highly left skewed, indicating that most genes are under purifying selection. (B) dN/dS estimates for genes grouped after GO categories. The GO categories are: Transcription (TS); Translation (TR); Transport (TP); Signal transduction (ST); Regulation of biological process (RP); Protein Transport (PT); Protein metabolic process (PP); Primary metabolic process (PM); Nucleic acid metabolic process (NM); Metabolic process (MP); Carbohydrate metabolic process (CM); Biosynthetic process (BP). Five GO categories were significantly different using a Mann-Whitney test when compared to all other genes: translation; protein metabolic process; nucleic acid metabolic process; primary metabolic process; transport. (C) Distribution of dN/dS estimates in six Giardia developmental stages and one category for constitutively expressed genes. The category labels represent: EX = Excystation; DI = Differentiation; EN = Encystation; CE = Cyst and excystation; TE = Tropohozoite and encystation; D = Genes down-regulated during cyst and excystation; CS = Constitutively expressed genes. Four categories were found to be significantly different when compared to all other groups using a Mann-Whitney test: constitutively expressed genes; trophozoite/encystation; cyst/excystation; differentiation. The categorization is based upon publicly available SAGE data (GEO GSE8336).

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