Increased expression of GNAi2 and APP in the PVN of the hypothalamus of stressed DBA/2J mice. A. Real-time PCR analysis shows an increase of GNAi2 mRNA in the PVN region of the hypothalamus of DBA/2J mice 4 h after stress. B. Real-time PCR analysis shows an increase in APP mRNA in the PVN 8 h after stress. C. Region-specific expression of GNAi2 and APP in the PVN of the hypothalamus of DBA/2J mice as shown by in situ hybridization. D. Real-time PCR analysis revealed no significant change of GNAi2 and APP in combined RNA samples from the hypothalamic region just anterior and posterior of the PVN (medial preoptic nucleus, partly from the dorsomedial hypothalamic nucleus and the periventricular hypothalamic nucleus).