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Table 1 Primers used in qPCR validation and the result from qPCR and microarray measurements.

From: Dynamic transcriptomic profiles of zebrafish gills in response to zinc depletion

  

Primers

Depletionb

Gene ID (symbol)a

Time point

Forward (5'-3')

Reverse (5'-3')

Array

qPCR

Array

qPCR

Dr.134300 (ppara)

24 h

GAACGCAACTGCAAGATCCAG

CCAAAACGAATAGCGTTGTGG

1.21 ± 0.5

0.99 ± 0.2

1.98 ± 0.2

1.52 ± 0.1

Dr.15390 (foxl1)

24 h

TGGTGTTTGGCATCCTCGAC

CTTGAAAGCGGTGTGTGAGTG

2.42 ± 1.0

0.97 ± 0.2

1.47 ± 0.4

0.84 ± 0.1

Dr.77183 (hmgcs1)

24 h

GTCACTCAAGATGGCACACCTG

CTCTGAGCTTCATTGTCTCAGC

1.36 ± 0.1

1.31 ± 0.4

1.57 ± 0.8

1.46 ± 0.8

Dr.76570 (apob)

24 h

TCAGCAATGCTATTCGCATGAG

CACTTATCCCATCACTCCGAGC

1.36 ± 0.5

1.04 ± 0.8

2.25 ± 0.6

2.06 ± 1.0

Dr.80336 (cyp11a1)

7d

GCAGCCCTGAAAGAAACTCTCA

TCCCTGCTGGAATGTGGTAATT

0.72 ± 0.4

2.72 ± 1.0

0.53 ± 0.2

0.41 ± 0.1

Dr.429 (dlx5)

7d

ACGCGAGCTCTTCGTGGTATT

CAGTACAACGTTCCTGATCCGA

0.38 ± 0.1

0.56 ± 0.2

0.56 ± 0.3

0.96 ± 0.5

Dr.2 (dlx2)

7d

GCCTCACGCAAACACAGGTT

AGACTCACCGGAGGCCACAT

1.08 ± 0.6

1.20 ± 0.5

1.87 ± 0.6

2.28 ± 1.2

Dr.78697 (rfx2)

14d

CCGGAGATCATCAGCACTAAGG

GGTCGCTGAGCATCTGATTGA

1.26 ± 0.8

0.75 ± 0.1

2.07 ± 0.3

2.99 ± 1.5

Dr.121290 (mt)

8 h

AATGGACCCCTGCGAAT

GGTAGCACCACAGTTGCAA

0.32 ± 0.3

0.30 ± 0.1

1.91 ± 1.5

1.87 ± 1.2

 

24 h

  

0.21 ± 0.2

0.81 ± 0.4

3.67 ± 0.6

2.72 ± 0.9

 

4d

  

0.53 ± 0.2

0.44 ± 0.2

4.90 ± 1.7

4.91 ± 2.9

 

7d

  

0.15 ± 0.1

0.38 ± 0.1

7.48 ± 1.6

5.65 ± 1.4

 

14d

  

0.91 ± 0.7

0.28 ± 0.1

13.82 ± 3.3

12.52 ± 4.4

Dr.12303 (znt1)

8 h

AGTGCCCGAGCAGATCGA

GCTAGAACTCCATCCAGGCTCTT

0.74 ± 0.2

0.82 ± 0.1

1.11 ± 0.0

1.30 ± 0.4

 

24 h

  

0.60 ± 0.4

0.94 ± 0.6

1.42 ± 0.4

1.11 ± 0.4

 

4d

  

0.89 ± 0.0

0.78 ± 0.2

0.88 ± 0.2

1.22 ± 0.2

 

7d

  

0.98 ± 0.0

0.55 ± 0.2

2.01 ± 0.6

1.57 ± 0.4

 

14d

  

0.92 ± 0.3

0.96 ± 0.4

1.35 ± 0.6

2.71 ± 0.9

18s

 

CGGAGGTTCGAAGACGATCA

CGGGTCGGCATCGTTTAC

    
  1. aGene IDs are provided as UnigeneID and ZNC gene symbols
  2. bExpression levels are expressed as mean ± SD of fish treated with zinc depletion or supplementation compared to control as analysed by the two methods for the durations indicated
  3. cSupplementation was conducted as a parallel experiment [30].