Sequence and transcriptional analysis of miRNAs dominantly expressed in S. japonicum. A. Alignment of sja-mir-7 sequences with homologues from other organisms. The seed sequences are shadowed in a dark colour. B. Northern-blot hybridization using probes complementary to the dominant miRNAs expressed during the adult worm stage. The 70 nt pre-miRNAs were indicated by arrows. C. Quantification analysis, relative to that in the egg stage, of miRNAs dominantly expressed in four developmental stages as indicated in different colours.