Physiological changes of zebrafish after zinc supplementation. Panel A: Elemental composition of whole body (minus gill) determined by ICP-MS. Panel B: Unidirectional Zn2+ influx across the gills. Zebrafish treated with zinc supplementation in feed and water for 14 days compared with fish kept at the standard control condition. The bars represent the mean of nine fish for elemental composition or eight fish for Zn2+ influx analysis; error bars denote SD. A statistical difference between zinc supplementation group and the control is shown by an asterisk (P < 0.05).