Molecular interactions between zinc, copper, iron, calcium and proteins encoded by transcripts changed by zinc supplementation. A Direct Interaction Network was automatically generated based on curated interactions contained within the proprietary PathwayArchitect database. An interactive version of this network is also provided (Additional file 2, Figure S1). Ovals represent proteins and the circles symbolize metal ions. Objects are coloured by their abundance in zebrafish at the time-point they were significantly different from the control is a scale from -4 fold (dark green) to +4 fold (dark red). Where significant differences were found at more than one time-point, the colour overlay shows expression at the first instance. Dark blue squares denote 'binding', and light blue squares 'expression'; green squares stand for 'regulation', green diamonds for 'metabolism', and green circles for 'promoter binding'. Arrow heads indicate directionality of the interaction where annotated.