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Table 3 Confirmation of microarray results by qPCR

From: Mating alters gene expression patterns in Drosophila melanogaster male heads

Gene identifier

Gene symbol

Microarray Fold change

qPCR Relative fold change ± SEM

Avg. relative expression level in control male heads ± SEM

Avg. relative expression level in mated male heads ± SEM

CG5618

 

1.14

2.02 ± 0.49*

0.36 ± 0.09

0.74 ± 0.18

CG6188

 

1.64

1.94 ± 0.26*

2.25 ± 0.42

4.38 ± 0.58

CG8449

 

1.28

1.35 ± 0.15*

1.24 ± 0.26

1.68 ± 0.18

CG16772

 

1.5

4.07 ± 1.55

6.86 ± 1.72

27.94 ± 10.61

CG30026

 

1.42

2.23 ± 0.35*

4.36 ± 0.84

9.74 ± 1.53

CG4501

bgm

1.38

4.47 ± 1.11*

1.42 ± 0.31

6.37 ± 1.57

CG6342

Irp-1B

1.26

1.42 ± 0.12

1.09 ± 0.18

1.55 ± 0.13

CG11765

Prx2540-2

1.2

1.23 ± 0.12*

0.47 ± 0.08

0.58 ± 0.06

CG2505

αEst2

-1.3

-1.26 ± 0.15

2.84 ± 0.59

2.25 ± 0.42

CG7390

smp-30

-1.36

-1.69 ± 0.16*

1.28 ± 0.45

0.77 ± 0.2

  1. * Indicates a significant (p < 0.05) difference between the average relative expression level in control male heads and mated male heads. SEM = Standard error of the mean.
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BMC Genomics

ISSN: 1471-2164

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