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Table 3 Confirmation of microarray results by qPCR

From: Mating alters gene expression patterns in Drosophila melanogaster male heads

Gene identifier Gene symbol Microarray Fold change qPCR Relative fold change ± SEM Avg. relative expression level in control male heads ± SEM Avg. relative expression level in mated male heads ± SEM
CG5618   1.14 2.02 ± 0.49* 0.36 ± 0.09 0.74 ± 0.18
CG6188   1.64 1.94 ± 0.26* 2.25 ± 0.42 4.38 ± 0.58
CG8449   1.28 1.35 ± 0.15* 1.24 ± 0.26 1.68 ± 0.18
CG16772   1.5 4.07 ± 1.55 6.86 ± 1.72 27.94 ± 10.61
CG30026   1.42 2.23 ± 0.35* 4.36 ± 0.84 9.74 ± 1.53
CG4501 bgm 1.38 4.47 ± 1.11* 1.42 ± 0.31 6.37 ± 1.57
CG6342 Irp-1B 1.26 1.42 ± 0.12 1.09 ± 0.18 1.55 ± 0.13
CG11765 Prx2540-2 1.2 1.23 ± 0.12* 0.47 ± 0.08 0.58 ± 0.06
CG2505 αEst2 -1.3 -1.26 ± 0.15 2.84 ± 0.59 2.25 ± 0.42
CG7390 smp-30 -1.36 -1.69 ± 0.16* 1.28 ± 0.45 0.77 ± 0.2
  1. * Indicates a significant (p < 0.05) difference between the average relative expression level in control male heads and mated male heads. SEM = Standard error of the mean.