Figure 1

Average length distribution of assemblies and pyrosequencing reads of the rainbow trout ESTs. Average lengths of the combination assembly contigs are more than those of the 454-pyrosequencing assembly over the 100-700 bp length range, indicating that addition of the Sanger-based data improved assembly of the short 454-pyrosequenceing reads up to 700 bp. However, over the 700-2000 bp length range, average contig lengths of the combination assembly are less than those of the 454-pyrosequencing assembly indicating that contigs longer than 700 bp of the combination assembly were mainly derived from the 454-pyroseqeuncing data, possibly because most of the 454-pyrosequencing reads were 400-600 bp. Number of the 454-pyrosequencing reads is divided by 10 for scaling.