Skip to main content

Table 2 Summary statistics of the Sanger-based assembly, 454-pyrosequencing assembly and a combination assembly of both datasets

From: Characterization of the rainbow trout transcriptome using Sanger and 454-pyrosequencing approaches

  Combination assembly Sanger assembly 454 assembly
Total sequences 521 M bases 74 M bases 447 M bases
Total no. of ESTs 1,380,311 258,973 1,290,292
No. of unique sequences 422,889 90,019 376,238
No. of sequences assembled 1,119,240 (81%, Ave = 394 bp) 209,565 (81%) 1,065,901 (83%, Ave = 374 bp)
No. of sequences unassembled 261,071 (Ave = 417 bp) 49,408 224,391 (Ave = 345 bp)
Total no. of contigs 161,818 (Ave = 758 bp) 40,320 (Ave = 999 bp) 151,847(Ave = 662 bp)
Total no. of large contigs (>500 bp) 101,464 78,572 84,810