Table 2 Summary statistics of the Sanger-based assembly, 454-pyrosequencing assembly and a combination assembly of both datasets
From: Characterization of the rainbow trout transcriptome using Sanger and 454-pyrosequencing approaches
| Â | Combination assembly | Sanger assembly | 454 assembly |
|---|---|---|---|
Total sequences | 521 M bases | 74 M bases | 447 M bases |
Total no. of ESTs | 1,380,311 | 258,973 | 1,290,292 |
No. of unique sequences | 422,889 | 90,019 | 376,238 |
No. of sequences assembled | 1,119,240 (81%, Ave = 394 bp) | 209,565 (81%) | 1,065,901 (83%, Ave = 374 bp) |
No. of sequences unassembled | 261,071 (Ave = 417 bp) | 49,408 | 224,391 (Ave = 345 bp) |
Total no. of contigs | 161,818 (Ave = 758 bp) | 40,320 (Ave = 999 bp) | 151,847(Ave = 662 bp) |
Total no. of large contigs (>500 bp) | 101,464 | 78,572 | 84,810 |