The adult female A. gambiae salivary gland transcriptome and its regulation upon blood-feeding. Genes that correspond to replica spots with a mean salivary gland RNA probe fluorescence hybridization intensity value >100 were grouped into the following three categories: (A) highly abundant transcripts (n = 267), with fluorescence values from 5,000 to 65,000; (B) moderately present transcripts (n = 482), with fluorescence values from 1,000 to 4,999; and (C) weakly present transcripts (n = 4,229), with fluorescence values from 100 to 999. The three categories were further sub-grouped into 11 functional groups according to their predicted functions, as shown in the pie charts. (D) The bar graph shows the number of genes that were regulated upon blood-feeding in different functional groups; those that were up-regulated (52 genes) are above zero (indicated by a red arrow), and those that were down-regulated (41 genes) are below zero (indicated by a green arrow). The color code for each group is shown. Those genes belonging to the unknown functional group are not shown in any of the diagrams.