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Table 1 Genes identified to be required for intracellular replication of L. monocytogenes within epithelial cells

From: Deciphering the intracellular metabolism of Listeria monocytogenes by mutant screening and modelling

    fold reduction     fold reduction
gene name or number protein name, or protein homology/similarity to polar effect intrac. invasion/adhesion gene name or number protein name, or protein homology/similarity to polar effect intrac. invasion/adhesion
cell wall      protein modification    
1lmo0441 penicillin-binding protein (D-alanyl-D-alanine carboxypeptidase) no 9.4 6.1 5lmo0618 protein kinase no 11.0 2.0
2,8lmo1085 teichoic acid biosynthesis protein B rf 12.3 2 1lmo0763 hypothetical Ser/Thr protein phosphatase family protein possible 5.5 2.0/5.0
lmo1088 teichoic acid biosynthesis protein B rf 26.2 1.9 metabolism of amino acids and related molecules    
7lmo1713 actin-like ATPase involved in cell morphogenesis no 3.6 3.5 lmo0594 homoserine O-acetyltransferase no 7.7 3.9
3,5,7pbpB (lmo2039) penicillin-binding protein 2B rf 3.8 2.6 6,8lmo1235 aspartokinase II a subunit no 5.9 nd
1lmo2555 Glycosyltransferase rf 8.1 3.9/5.0 lmo1495 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase possible 6.2 1.0
transport/binding proteins and lipoproteins     lmo1916 peptidase no 7.0 2.4
4lmo0135 (ctpA) oligopeptide ABC transport system, substrate binding protein rf 13.6 2.2 8aroB (lmo1927) 3-dehydroquinate synthase rf 2.8 1.4
4lmo0136 oligopeptide ABC transport system, substrate binding protein rf 5.0 2.4 4,6ilvB (lmo1484) acetolactate synthase (acetohydroxy-acid synthase) rf 5.5 1.8
lmo0137 oligopeptide ABC transport system, permease no 9.7 1.9 4,6ilvC (lmo1486) ketol-acid reductoisomerase no 3.2 3
4lmo0195 ABC-type antimicrobial peptide transport system, permease no 6.3 nd lmo2051 weakly similar to proteases no 4.1 1
lmo0495 permease of the drug/metabolite transporter (DMT) superfamily possible 4.2 2.3 lmo2694 lysine decarboxylase possible 5.5 0
4lmo0584 conserved hypothetical membrane protein, putative permease no 7.2 3.0 lmo2770 γ-glutamylcysteine synthetase and cyanophycin synthetase no 5.8 2.3
lmo0645 amino acid transporter no 4.9 1.5 6serC (lmo2825) phosphoserine aminotransferase possible 5.7 3.5
5lmo0650 conserved membrane protein possible 2.8 2.0 metabolism of nucleotides and nucleic acids    
lmo0787 amino acid transporter no 3.4 1.0 1,5purA (lmo0055) adenylosuccinate synthetase no 14.9 /4.0
lmo0810 spermidine/putrescine-binding protein no 7.4 3.1 purQ (lmo1769) phosphoribosylformylglycinamidine synthetase rf 12.0 nd
3lmo1003 phosphotransferase system enzyme I no 3.4 1.0 8purS (lmo1771) phosphoribosylformylglycinamidine synthetase rf 11.0 1.0
gbuA (lmo1014) glycine betaine ABC transporter, ATP-binding protein rf 10.0 4.0 pyrE (lmo1831) orotatephosphoribosyltransferase no 5.5 2.5
8lmo1416 hypothetical transporter no 5.3 1.0 metabolism of lipids    
6opuCA (lmo1428) glycine betaine/carnitine/choline ABC transporter, ATP-binding protein rf 15.7 1.7 5,8ispE (lmo0190) CDP-ME synthase involved in isoprenoid biosynthesis no 4.7 2.3
5lmo1431 ABC transporter, ATP binding protein no 4.8 3 lmo1005 3-hydroxyisobutyrate dehydrogenase no 11.0 1.8
lmo1506 ABC-type antimicrobial peptide transport system, permease rf 6.2 2.3 lmo1363 geranyltransferase no 5.0 2.8
lmo1739 amino acid ABC transporter rf 5.5 2 lmo2450 carboxylesterase possible 4.2 3.0
3,5,7,8lmo1847 ABC transporter specific for metal cations no 5.8 2.3 metabolism of coenzymes and prosthetic groups    
lmo2124 maltodextrin ABC transport system, permease rf 3.4 0 5lmo0221 hypothetical type III pantothenate kinase possible 6.9 1.0
1,3oppF (lmo2192) oligopeptide ABC-transporter, ATP-binding protein no 21.0 8.0/4.0 1,3pdxK (lmo0662) pyridoxine kinase no 13.1 1/4.0
lmo2227 ABC transporter, ATP-binding protein possible 5.8 1.3 lmo1043 molybdopterin-guanine dinucleotide biosynthesis MobB rf 7.9 1.4
lmo2249 low-affinity inorganic phosphate transporter no 2.8 2.0 lmo1932 heptaprenyl diphosphate synthase component I rf 12.1 4.8
lmo2353 hypothetical Na+/H+ antiporter no 4.3 2.3 nadB (lmo2023) L-aspartate oxidase rf 3.4 0
lmo2380 protein involved in resistance to cholate/Na+ and in pH homeostasis rf 5.7 2.5 3lmo2102 glutamine amidotransferase subunit PdxT (pyridoxine biosynthesis) no 4.0 3.3
lmo2430 B. subtilis ferrichrome ABC transporter FhuG, permease rf 75.6 5.0 lmo2566 biotin/lipoate A/B protein ligase family possible 6.2 2.0
lmo2816 transport protein no 6.3 2.0 DNA restriction/modification and repair    
lmo2850 sugar transport protein rf 16.5 0 5gyrB (lmo0006) DNA gyrase subunit B rf 9.6 nd
sensors      lmo0157 ATP-dependent helicase possible 2.1 1.0
lmo0799 oxygen/light sensor with PAS domain no 8.3 2.0 mfd (lmo0214) transcription-repair coupling factor possible 8.9 3.7
lmo1508 two-component sensor histidine kinase no 5.2 2.9 lmo0588 DNA photolyase no 9.6 2.0
membrane bioenergetics     mutM (lmo1564) formamidopyrimidine-DNA glycosylase no 5.2 1.1
lmo0091 ATP synthase g chain, H+-transporting two-sector ATPase rf 5.7 2.0 lmo1751 hypothetical RNA methyltransferase no 6.2 2.0
nifJ (lmo0829) pyruvate-flavodoxin oxidoreductase no 6.4 3.6 8ansB (lmo1663) asparaginyl-tRNA synthetases no 4.1 1.6
5atpA (lmo2531) H+-transporting ATP synthase chain α rf 7.7 2.7 lmo2050 exconuclease ABC (subunit A) no 5.1 2.0
atpB (lmo2535) H+-transporting ATP synthase chain β rf 4.4 5.9 DNA recombination    
mobility and chemotaxis     6recN (lmo1368) RecN no 14.8 1.9
lmo0680 flagella-associated protein FlhA rf 7.0 3.1 ruvB (lmo1532) Holliday junction DNA helicase RuvB possible 9.4 3.9
6lmo0700 flagellar motor switch protein FliY rf 5.8 1.7 8recS (lmo1942) similar to ATP-dependent DNA helicase no 7.0 2.3
cell surface proteins     regulation     
1,2,6vip (lmo0320) putative peptidoglycan bound protein with LPXTG motif no 6.4 4.5 3agrA (lmo0051) 2-component response regulator protein no 3.4 1.0
8lmo0327 protein with LPXTG motif, putative murein hydrolase activity no 6.9 3.0 6lmo0294 transcription regulator, LysR-gltR family no 7.4 3.1
4lmo0514 internalin-like protein with LPXTG motif no 6.4 1.4 lmo0535 transcription regulator, LacI family no 9.5 1.0
4lmo0576 hypothetical cell wall associated protein no 4.2 1.5 3fur (lmo1956) transcriptional regulator, Fur family no 5.5 1.0
2,8lmo1666 peptidoglycan linked protein with LPXTG motif no 6.8 1.8 lmo1994 transcription regulators, LacI family no 9.0 1.4
lmo2026 hypothetical peptidoglycan bound protein with LPXTG motif no 8.7 1.4 RNA modification     
5lmo2504 cell wall binding protein, peptidase-related enzyme no 4.8 2.0 lmo0241 hypothetical RNA methyltransferase, trmH family protein rf 4.1 1.0
1ami (lmo2558) autolysin, N-acetylmuramoyl-L-alanine amidase no 3.8 5.3 1,3,5lmo1434 RNA-metabolising metallo-β-lactamase no 9.6 3/22
metabolism of carbohydrates and related molecules     miscellaneous     
glpQ (lmo0052) transmembrane protein with phosphoesterase domain possible 4.1 2.0 lmo0066 toxin component of A/B toxin rf 2.8 1.0
8lmo0182 α-xylosidase and α-glucosidase rf 7.9 1.3 4lmo0585 secreted protein no 4.8 1.0
lmo0261 phospho-β-glucosidase no 3.4 0 lmo0587 secreted protein, YapH from Y. pestis, cell wall surface protein no 3.4 1.0
8lmo0271 phospho-β-glucosidase possible 4.1 1.0 unknown proteins     
lmo0517 phosphoglycerate mutase no 13.8 2.3 lmo0276 hypothetical hydrolase, HAD superfamily possible 5.2 1.3
2lmo1031 hypothetical L-fucose isomerase rf 11.1 2.1 2lmo0313 hypothetical hydrolase, PHP superfamily no 2.8 1.0
2lmo1032 Transketolase rf 14.4   4lmo0590 hypothetical DAK2/DegV domain-containing protein possible 9.6 1.0
lmo1166 NADPH-dependent butanol dehydrogenaseI possible 4.2 1.0 lmo0765 unknown protein possible 10.4 3.0
4glpD (lmo1293) glycerol-3-phosphate dehydrogenase no 4.8 1.0 4,6,7lmo0794 B. subtilis YwnB protein no 12.4 2.0
6lmo1244 weakly similar to phosphoglycerate mutase 1 no 8.3 2.0 lmo1379 B. subtilis SpoIIIJ protein, hypothetical membrane protein no 4.8 3.0
lmo2005 Oxidoreductase no 7.4 3.9 3lmo1402 B. subtilis YmcA protein no 2.1 2.0
lmo2015 α-mannosidase no 6.4 1.7 lmo1575 phosphoesterase, DHH superfamily no n.d. 1.0
lmo2134 fructose-1,6-biphosphate aldolase type II no 5.2 2.4 6,8lmo1700 unknown protein no 4.6 1.3
lmo2172 propionate CoA-transferase possible 5.3 1.3 4lmo1830 short chain dehydrogenase no 11.7 2.0
lmo2247 Oxidoreductase possible 5.0 2.4 lmo1866 hypothetical phosphotransferase possible 7.1 2.2
8lmo2446 Glycosidase no 9.9 2.5 lmo1920 unknown protein   16.5 1.0
lmo2586 formate dehydrogenase α-chain possible 2.4 2.2 lmo2516 conserved hypothetical protein possible 3.6 1.2
6lmo2660 Transketolase rf 3.0 2.6 lmo2639 unknown protein, contains DUF1312 domain possible 5.5 1.0
lmo2764 xylose operon regulatory protein and to glucose kinase rf 3.6 2.1 no similarity     
2lmo2781 β-glucosidase rf 6.5 2.0 lmo0729 no similarity no 4.8 1.0
lmo2831 Phosphoglucomutase no 5.3 2.8 2lmo1188 no similarity no 5.1 3.5
      lmo1219 no similarity no 4.8 2.0
      lmo2129 no similarity no 5.8 1.6
  1. 1adhesion (seven genes) or invasion (four genes) defective as determined 35 min or 2 h post infection, respectively; 2genes without homologue in L. innocua; genes downregulated3 or upregulated4 during growth in macrophages [11]; genes downregulated5 or upregulated6 during growth in Caco-2 cells [12]; 7induced in vivo [23]; 8prfA binding box is located upstream [13]. Fold reduction of intracellular (intrac.) replication in comparison to a control group of >100 insertion mutants [12]. rf, related function of downstream genes. Four genes from categories which one gene only (1.6 protein secretion, 1.7 cell division, 1.10 transformation, and 3.7 protein synthesis) was assigned to are not shown. See also Additional file 6 for generation times.