Microsatellites border the conserved sequence flanking the Sp185/333 genes. A. Pairwise diversity of Sp185/333 genes and flanking regions. Pairwise diversity was calculated among five regions (gene and four flanking regions) in MEGA . The Sp185/333 gene (on the x-axis in red; 5' to 3' orientation) represents a generic gene with the intron shown as a thinner region. The flanking regions are defined by the edge of the gene and the location of the GA microsatellites (purple triangles). Region 1 (~250 nt; dark gray) is upstream of the 5' GA microsatellite. Region 2 (~430 nt; light gray) is between the GA microsatellite and the start codon. Region 3 (~330 nt; light gray) is between the stop codon and the 3' GA microsatellite. Region 4 (~330 nt; dark dray) is downstream of the 3' GA microsatellite. The two colors in each line correspond to the two genes that were used in the pairwise comparison and match the gene colors shown in Figure 4. Three categories of pairwise diversity are i) high (squares) in regions flanking the gene, ii) low (circles) in all regions including the gene sequences, and iii) hybrid (triangles) where pairwise diversity is low in regions 1 and 2 and high in regions 3 and 4. B. The microsatellites are boundaries for sequence conservation. Alignments of the genes and flanking sequences were used to calculate the entropy over a 30 nt window that slides 1 nt for each calculation. Entropy scores are shown for the analysis with all six genes (blue line) and for only the three D1 genes (red line). The black lines show the average diversity of the regions indicated on the x-axis for all six genes (dashed line), or only the three D1 genes (dotted line).