The distribution of TFBSs in yeast promoters. (A) The frequency distribution of the distances from the TFBSs to the transcription starting site (TSS) in genes. This figure shows the spatial distribution of TFBSs in the promoters of 4369 genes (using TFBS dataset IV). The value at each position relative to the TSS is a moving average of a window of 41 bp. The distribution has a very sharp peak ~115 bp upstream of the TSS and the TFBSs are strongly concentrated in the ~ 100 bp region from 180 to 80 bp upstream of the TSS. The blue solid line represents observed values; The red solid and dotted lines represent the mean of randomization and 95% confidence intervals for 1000 randomized tests (B) No sharp peak was found in the frequency distribution of TFBSs relative to the translation start codon. This figure was generated using the same data as in (A) except that the translation start codon instead of the TSS was used as the reference point. (C) The frequency of deletion polymorphisms in the TSS-proximal region is the lowest in the promoter region and is significant lower than random expectation. This figure was generated using the same data as in (A).