Analysis by quantitative PCR (qPCR) of CNVR22 ( SLC16A7 gene). Twenty-one animals with statistical evidence for CNVR, three false negative and two Iberian from the control group are plotted. The horizontal dashed line represents the relative quantification (RQ) value of the reference animal. Each dot represents the relative copy number of the animal in comparison to the reference. Y-axis shows the RQ value obtained by qPCR. Vertical bars represent the standard error. The vertical dashed line separates the 17 related IBMAP individuals from the nine unrelated American local breeds. Breed abbreviations as described in Figure 3.