Real time PCR quantification of mRNA levels of selected transcriptional regulators differentially regulated at 7 versus 35 days. A-E, levels of mRNA for CREBBP, Atf5, Ankrd 1, Ankrd 2 and LIMD 1 are shown. Groups contained data for 7 to 8 animals each. Data are means ± SEM and were analyzed by one-way ANOVA; p values for the F statistic are shown under each panel. *, p < 0.05, **, p < 0.01, ***, p < 0.001 versus the indicated group.