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Table 3 KL3 genome annotation

From: Genomic analysis and relatedness of P2-like phages of the Burkholderia cepacia complex

Gene

Start

End

Putative function

Strand

Predicted RBS and start codon

Length (no. of aa residues)

Closest relative

Alignment region (no. of aa residues)

% ID

Source

GenBank accession no.

1

122

1150

integrase

-

GGCGCAGtgtgATG

342

integrase family protein

1-342/342

98

Burkholderia ambifaria MEX-5

ZP_02905720.1

2

1150

1416

unknown

-

GAAAAtcaccATG

88

hypothetical protein BamMEX5DRAFT_1075

1-88/88

97

Burkholderia ambifaria MEX-5

ZP_02905721.1

3

2096

4891

zinc finger CHC2-family protein

-

AACAGCAAtaacgATG

931

zinc finger CHC2-family protein

1-931/931

95

Burkholderia ambifaria MEX-5

ZP_02905722.1

4

4894

5142

unknown

-

GGAGGcgcagcagcATG

82

conserved hypothetical protein

1-82/84

91

Burkholderia ambifaria MEX-5

ZP_02905723.1

5

5139

5501

unknown

-

GCGGGGctgacacgATG

120

conserved hypothetical protein

1-120/120

97

Burkholderia ambifaria MEX-5

ZP_02905724.1

6

5506

5700

membrane protein

-

GGAAccacaccATG

64

putative phage-encoded membrane protein

1-64/64

93

Burkholderia ambifaria MEX-5

ZP_02905725.1

7

5744

5938

unknown

-

GCACTGAtccgATG

64

hypothetical protein bglu_1 g03740

1-64/64

95

Burkholderia glumae BGR1

YP_002910278.1

8

5943

6155

unknown

-

GAAAAAAGGAGAtcagcATG

70

conserved hypothetical protein

1-70/70

72

Burkholderia sp. CCGE1001

ZP_06292843.1

9

6243

6491

transcriptional activator (Ogr)

-

GGAGTAAGccgaaATG

82

putative phage transcriptional activator Ogr/Delta

1-82/82

92

Burkholderia glumae BGR1

YP_002910024.1

10

6479

6682

unknown

-

AATGAGTAGctcctacgATG

67

hypothetical protein BoklE_00724

1-67/67

85

Burkholderia oklahomensis EO147

ZP_02353966.1

11

6722

6919

unknown

-

GAGGAGcccgcATG

65

hypothetical protein BoklE_00729

1-65/65

87

Burkholderia oklahomensis EO147

ZP_02353967.1

12

6934

7143

unknown

-

AAAGTATAccgaccATG

69

hypothetical protein BoklE_00734

1-62/71

87

Burkholderia oklahomensis EO147

ZP_02353968.1

13

7193

7732

repressor

+

GGTAAGGctagtgtaATG

179

hypothetical protein BCAL0086

1-163/163

62

Burkholderia cenocepacia J2315

YP_002229252.1

14

7904

8179

unknown

+

GAGGGAccagaagaATG

91

hypothetical protein BuboB_27112

1-91/98

50

Burkholderia ubonensis Bu

ZP_02381422.1

15

8238

9329

tail protein (D)

-

GGACGCGGAGccgaaggcATG

363

fels-2 prophage protein

19-366/366

81

Burkholderia oklahomensis EO147

ZP_02353972.1

16

9326

9784

tail protein (U)

-

ACGGAGGAtctgtcccATG

152

bacteriophage tail-related protein

1-133/141

66

Burkholderia oklahomensis EO147

ZP_02353973.1

17

9806

13033

tail tape measure protein (T)

-

GAAGCGGAcacgagtaacgATG

1075

hypothetical protein bglu_1 g01240

1-1079/1079

59

Burkholderia glumae BGR1

YP_002910030.1

18

13158

13508

tail protein (E)

-

AGGACACGcaacatATG

116

gpE+E'

1-114/114

76

Burkholderia pseudomallei 112

ZP_02501899.1

19

13036

13508

tail protein (E+E')

-

AGGACACGcaacatATG

157

gpE+E'

1-100/114

74

Burkholderia pseudomallei 112

ZP_02501899.1

20

13578

14087

tail tube protein (FII)

-

AGGAGtcacacacATG

169

phage major tail tube protein

1-169/169

74

Burkholderia cenocepacia J2315

YP_002229260.1

21

14103

15275

tail sheath protein (FI)

-

AGGAGctgcacaccATG

390

phage tail sheath protein

1-390/390

84

Burkholderia pseudomallei 1655

ZP_04890547.1

22

15328

15951

tail fiber assembly protein

-

ACGGAcctcgaaacATG

207

tail fiber assembly protein from lambdoid prophage e14

1-190/209

85

Burkholderia ubonensis Bu

ZP_02381414.1

23

15969

18632

tail fiber protein

-

GGATAcctgaacATG

887

putative phage tail protein

1-883/883

71

Burkholderia cenocepacia J2315

YP_002229263.1

24

18635

19189

baseplate assembly protein (I)

-

GATGGCGGGGtcgcggATG

184

phage-related tail protein

1-183/184

84

Burkholderia pseudomallei 7894

ZP_02487653.1

25

19182

20087

baseplate assembly protein (J)

-

GAACGGAGtcggcgcATG

301

baseplate J-like protein

1-301/301

90

Burkholderia thailandensis E264

YP_439531.1

26

20084

20446

baseplate assembly protein (W)

-

GGAGCGGtgcATG

120

phage baseplate assembly protein

1-117/120

78

Burkholderia pseudomallei 7894

ZP_02487655.1

27

20443

21138

baseplate assembly protein (V)

-

GAGGGCGGccggcaacATG

231

phage baseplate assembly protein

33-261/261

72

Burkholderia phage Ï•52237

YP_293735.1

28

21304

22080

N-4/N-6 DNA methylase

+

ACGTTGcctcagaaccATG

258

site-specific DNA methyltransferase

34-290/291

78

Burkholderia pseudomallei K96243

YP_111089.1

29

22060

22527

tail completion protein (S)

-

GAGCAATGGGtggcgtgATG

155

phage virion morphogenesis protein

1-155/155

87

Burkholderia thailandensis MSMB43

ZP_02466375.1

30

22527

22943

tail completion protein (R)

-

AGACGGccgcccATG

138

bacteriophage tail completion protein R

1-138/138

73

Burkholderia pseudomallei K96243

YP_111086.1

31

22936

23220

Rz1 (LysC)

-

GGAGActcatcgATG

94

hypothetical phage protein

1-83/91

75

Burkholderia pseudomallei 1655

ZP_04890536.1

32

23057

23497

Rz (LysB)

-

GAAGGcggccgcGTG

146

protein lysB

1-146/146

62

Burkholderia thailandensis E264

YP_439538.1

33

23494

24303

endolysin

-

GGAGCAccgaatcATG

269

putative phage-encoded peptidoglycan binding protein

1-269/270

73

Burkholderia pseudomallei K96243

YP_106791.1

34

24300

24572

holin

-

AGGGGGAAAtgacATG

90

protein of unknown function DUF754

1-88/88

67

Burkholderia sp. CCGE1001

ZP_06292819.1

35

24574

24918

putative antiholin

-

GGAAttgtccgaATG

114

hypothetical protein Bpse38_23639

1-113/114

83

Burkholderia thailandensis MSMB43

ZP_02466369.1

36

24934

25140

tail protein (X)

-

GGTTGAActgatctgATG

68

phage tail protein X

1-68/68

88

Burkholderia pseudomallei 7894

ZP_02487665.1

37

25140

25619

capsid completion protein (L)

-

GAATCGaccATG

159

fels-2 prophage protein

1-159/159

86

Burkholderia thailandensis E264

ZP_05590935.1

38

25719

26408

terminase endonuclease subunit (M)

-

GAGCTGGtggcggcATG

229

hypothetical protein bglu_1 g01450

1-228/229

95

Burkholderia glumae BGR1

YP_002910051.1

39

26405

27418

capsid protein (N)

-

GGAGAAcccaactcATG

337

Gp2, phage major capsid protein, P2 family protein

1-337/337

98

Burkholderia glumae BGR1

YP_002910052.1

40

27454

28266

capsid scaffolding protein (O)

-

GGTTCGAcctctctctATG

270

phage capsid scaffolding protein (GPO)

1-270/270

95

Burkholderia glumae BGR1

YP_002910053.1

41

28345

30180

terminase ATPase subunit (P)

+

AACGAGcggcgtATG

611

phage terminase, ATPase subunit

1-589/589

99

Burkholderia glumae BGR1

YP_002910054.1

42

30177

31226

portal vertex protein (Q)

+

GGAGttctattcATG

349

Gp5, phage portal protein, pbsx family protein

1-347/351

99

Burkholderia glumae BGR1

YP_002910055.1

43

31595

31732

unknown

+

GATGcgcgATG

45

none

    

44

31722

33290

unknown

-

GGGGAAAGcaacatATG

522

hypothetical protein ECO103_1901

1-526/527

54

E. coli O103:H2 str. 12009

YP_003221840.1

45

33455

34699

EcoRII-C endonuclease

-

AACGGAGcttcggggATG

414

type II restriction endonuclease, EcoRII-C domain protein

1-401/401

77

Candidatus Hamiltonella defensa 5AT (Acyrthosiphon pisum)

YP_002923978.1

46

34696

35142

Vsr endonuclease

-

TCGCctgATG

148

DNA mismatch endonuclease Vsr

1-148/148

77

Burkholderia graminis C4D1M

ZP_02883050.1

47

35142

36410

EcoRII DNA cytosine methylase

-

AGCGAGAGcaaatATG

422

DNA-cytosine methyltransferase

1-426/426

81

Burkholderia phytofirmans PsJN

YP_001894783.1

48

36570

37685

unknown

-

AAGCTGAcgctATG

372

conserved hypothetical protein

1-371/371

95

Burkholderia ambifaria MEX-5

ZP_02905764.1

49

37728

38816

unknown

-

AGTTctctaattgacATG

362

GP30 family protein

1-362/362

96

Burkholderia ambifaria MEX-5

ZP_02905765.1

50

38822

39526

unknown

-

GGGAGAAGcctgaATG

234

VRR-NUC domain protein

1-234/234

99

Burkholderia ambifaria MEX-5

ZP_02905766.1

51

39523

40032

unknown

-

AGGAGttcagcATG

169

PAAR repeat-containing protein

1-169/169

97

Burkholderia ambifaria MEX-5

ZP_02905767.1

52

40202

40408

transcriptional regulator

+

AAGGAGAAAtagcATG

68

phage transcriptional regulator, AlpA

1-68/72

94

Burkholderia ambifaria MEX-5

ZP_02905768.1

  1. Abbreviations: RBS, ribosome-binding site; aa, amino acid; % ID, percent identity. The P2 proteins that are similar to KL3 proteins based on CoreGenes analysis are shown in brackets in the putative function column.