The GH5 family of putative cell-wall-modifying enzymes. The tree includes GH5 proteins from 28 species: Arabidopsis (AT), rice, Brachypodium (Bradi), sorghum, and poplar, as well as 14 additional eudicot species, five additional monocot species, a gymnosperm, and three species of green algae. Tree construction and color-coding (blue for eudicots and red for monocots) are as in Figure 2. A light blue diamond indicates the single gymnosperm sequence; green squares indicate green algal sequences. The three major plant clades, A, B, and C, are marked with dotted, gray lines. Small gray arrows indicate HvMAN1 (Hor vul ABC87082.1), LeMAN4a (Sol lyc AAK97760.1), and GH5BG (Os10g0370500). For clarity, gene names or accession numbers are shown only for Arabidopsis, Brachypodium, the gymnosperm, and algae. Mic sp: Micromonas sp. RCC299; Ost luc: Ostreococcus lucimarinus; Ost tau: Ostreococcus tauri; Pic sit: Picea sitchensis. For complete branch labels and bootstrap values, see additional file 13.