The GH13 family of α-amylases, pullulanases, isoamylases, and branching enzymes. The tree includes GH13 proteins from Arabidopsis (AT), rice (Os), Brachypodium (Bradi), sorghum (Sb), and poplar (Poptr). Tree construction and color-coding (blue for eudicots and red for grasses) are as in Figure 2. The three major clades are marked with dotted, gray lines and labeled with enzymatic activities. For complete bootstrap values, see additional file 16.