A novel multifunctional oligonucleotide microarray for Toxoplasma gondii

BMC Genomics

Table 2 Gene Expression in Toxoplasma gondii.

	Number of:		Evidence for expression in tachyzoites:
	Probes/Tags¹	Genes (total = 7793)	Anystrain²	RH (type I)	Pru (type II)	VEG (type III)	No expression
EST studies	125,741	2,336	2,185 (2,073)	NA	NA	NA	5,608 (5,270)
SAGE tag studies	38,263	1,229	901 (488)	NA	NA	NA	6,892 (7,305)
Spotted cDNA arrays	2,449	501	204 (106)	NA	NA	NA	2,245 (2,343)
Photolithographic array	8,058 ³	7,793	3,986 (3,270)	3,395 (1,692)	3,065 (1,472)	3,185 (1,623)	4,072 (2,154)

1 Number of Toxoplasma ESTs in dbEST, unique SAGE tags in TgSAGEDB, and probesets on the microarrays.
2 Values in parentheses reflect a more stringent criteria for evidence of expression: > = 3 SAGE tags or ESTs vs. > = 1; 150% above background vs. > 0% above background; 5% FDR vs. 10% FDR for Affymetrix arrays.
3 Some genes have more than 1 associated probeset, differing in degree of potential cross hybridization.

ISSN: 1471-2164