Table 3 Primers and PCR product size of the genes used for semi-quantitative RT-PCR analysis

1.

Carboxyl transferase of ACCase β subunit (CT-β)

GAATGAGTTACTTCAGCTTCAC

AATTCACCCTTCTTTCTTGTTGA

307

2.

Biotin carboxyl carrier protein I of ACCase (BCCPI)

GTCGGCTAATCTTAAAGCTATTC

TGTTTATAGCTTCACTAGTGTAC

258

3.

Biotin carboxyl carrier protein II of ACCase (BCCPII)

GATGCTCTCATTGCAATTCTC

TAATGATAAATAACAATGAAGAAGG

278

4.

Malonyl-CoA:ACP transacylase (MCAT)

ACTTCTCCTGTTCAATGGGAA

TCTCTAGTACATGACGCTAATC

314

5.

3-keto acyl ACP Reductase (KAR)

GTTATCTCTCCCGAAAGTGTA

CACAGTATCTGTCACCTTTTC

306

6.

Beta-keto acyl ACP synthase I (KASI)

GCATCTGGCTTGTCTCCAT

GCAGAAGAACACAATTTTGATAC

402

7.

Beta-keto acyl ACP synthase II (KASII)

TGTTCCCAATTTGAAGAAGCAG

AGGAACACCAAATCCAATCTTAT

307

8.

Acyl carrier Protein (ACP)

AAGATCAGTCTAGGAAATCCTTC

GCATAAATTAGGAAATTTTAGAGTGT

180

9.

Acyl ACP thioesterase (FATA)

AATAATGTAGATTTCTTTATTTGTGT

AGTAAACGTAAAACAATACAGTTGAT

285

10.

ω-3-fatty acid Desaturase (ω-3-FAD)

AAAGCTGCAAAATTTTTTATCTGCA

CCCTCTCAAATCCAATCCAA

215

11.

Acyl -CoA binding protein (ACBP)

TGAACATTTCTATGCCGCTTG

GAGAAATAAGGGTCACCATTATC

310

12.

Long chain acyl-CoA synthetase (LCACS)

ATCCATAGCATTTGGCTTTCAAA

CGTGTAGAAGATGAATTGTATAAC

326

13.

Acyl-CoA thioesterase (ACT)

ACTCATCTTAGCTTTGTTATGTTC

CCCATTTCAATCACCGTTTC

173

14.

Glycerol-3-phosphate acyl transferase (GPAT)

GGGTAACGTGTTGGGATTTG

CACAGAATCCAAATAATTCTACATTT

200

15.

Lysophosphatidic acid acyl transferase (LPAT)

TGGTGTATGTTTGTGCTTGG

CTGTACAAAATTGAATCAAGCTTTTT

238

16.

Diacyl glycerol kinase (DGK)

CGGCTATTCGGTTGGAAATAA

GATTTTTGATACAACAAATTACCAGT

296

17.

Triacyl glycerol lipase(TAGL)

CTGGAGACAAGACGAGAATAA

CTTCTATCATAATCAATTATTGTTCG

310

18.

Actin

CAAGTCATCACCATTGGAGCA

GCCTCTTAATTTCGGCTTTAACA

590