Figure 1
From: Conserved and variable correlated mutations in the plant MADS protein network
Correlated mutation analysis of MADS domain proteins: workflow diagram. Sequences were obtained via blast, interpro and from a set of sequenced plant genomes. Orthologs were assigned using a best-hit criterium, followed by clustering to group sequences within each species with a very high sequence identity and filtering to remove sequences with low sequence identity to Arabidopsis MADS sequences. Intramolecular and intermolecular correlated mutations were obtained, and validated using various datasets. Subsequently, conservation of correlated mutations between proteins was analyzed, and correlated mutations were compared between interacting and non-interacting proteins. A. th. is Arabidopsis thaliana.