Figure 5

Effects of [BF/S+L/Ep] treatment on the trafficking activity of DCs. A, Two million BMDCs treated with DMSO (a-c), [BF/S+L/Ep] (d-f) and LPS (g-i) were labeled with green fluorescence dye and injected into allogeneic mice. Tissues of control BALB/c mice which were injected with DCs without fluorescence dye staining did not show significant green fluorescence spots, while the auto-fluorescence background was rather low (j-l). After 24 h mice were sacrificed and DC mobility compared by detecting DCs with green fluorescence in peripheral lymph nodes (a, d, g and j), spleen (b, e, h and k) and liver (c, f, I and l). Insets (d, e, g and h) show digitally amplified magnifications of treated DCs exhibiting different cellular behaviors in tissue trafficking and different levels in fluorescent intensity. Samples of liver tissue were detected as negative for fluorescence staining of DCs throughout the observation period.