Table 1 Summary statistics for pyrosequencing and annotation of P. margaritifera mantle ESTs

Total number of ESTs sequenced

276 738

-

Average lenght of ESTs (bp)

234

-

Number of assembled EST

219 205

79.2%

Number of contigs

19 257

-

Number of singletons

57 533

20.8%

Number of unique sequences

76 790

-

Ratio of singletons per unique sequences

-

74.9%

Number of contigs containing 2 ESTs

8 717

45.3%

Number of contigs containing 3 ESTs

3 419

17.8%

Number of contigs containing 4 ESTs

1 779

9.2%

Number of contigs containing 5 ESTs

1 119

5.8%

Number of contigs containing > 6 ESTs

4 223

21.9%

Number of annotated unique sequences:

29 479

38.4%

   - Known protein

13 064

44.3%

   - Uncharacterized

6 010

20.4%

   - Predicted

4 795

16.3%

   - Unknown, Unnamed

2 880

9.8%

   - Hypothetical protein

2 730

9.3%

Number of annotated contigs

10 007

52.2%

Number of annotated singletons

19 472

33.8%