Chromosome 16 in the lampbrush form. (a-a'') Centromere localisation (a) by immunostaining for cohesion subunit STAG2 (pink) followed by FISH mapping (a') of BAC clones WAG137G04 (NOR, green), WAG523B09 (MHC-Y complex, red) and WAG65G09 (MHC-B complex, yellow) on the same chromosome. Chromosomes are counterstained with DAPI (blue). (a") The schematic drawing shows the distribution of the signals. Grey circles indicate DAPI-stained chromomeres. (b-b'') High-resolution analysis of distribution of CNM (yellow), PO41 (pink) repeats and BAC clone WAG137G04 (NOR, green) (b'). (b) Corresponding DAPI image of the LBC16 is shown. (b") The schematic drawing shows the distribution of the signals. Scale bars = 5 μm. (c) Cytological map of GGA16 in the lampbrush form. Bright DAPI-positive chromomeres are represented by blue circles, the remaining chromomeres by white circles. The red circle indicates centromeric cohesion-enriched structure. The positions of CNM and PO41 repeats as well as NOR, MHC-Y and MHC-B complexes are shown on the right. Chiasma distribution along the bivalent is shown by the histogram on the left.