Skip to main content

Advertisement

Table 1 Summary of BAC libraries and HICF fingerprinting of BAC clones

From: Genomic tools development for Aquilegia: construction of a BAC-based physical map

Library Restriction digest Cloning vector Average insert size (kb) # clones Genome coverage Valid bands per clone (average) # of clones fingerprinted
AF_Bba Hind III pECBAC1 144.6 29,568 15.2 X 83.7 28,728
AF_Bca Mbo I pECBAC1 110.9 28,800 13.3 X 78.6 28,393
Total    128 58,368 28.5 X 81.1 57,121b
  1. aThe 2 libraries were constructed from A. formosa
  2. bAmong the 57121 clones were 50,155 clones used for FPC project