Genomic tools development for Aquilegia: construction of a BAC-based physical map

BMC Genomics

Table 1 Summary of BAC libraries and HICF fingerprinting of BAC clones

Library	Restriction digest	Cloning vector	Average insert size (kb)	# clones	Genome coverage	Valid bands per clone (average)	# of clones fingerprinted
AF_Bb^a	Hind III	pECBAC1	144.6	29,568	15.2 X	83.7	28,728
AF_Bc^a	Mbo I	pECBAC1	110.9	28,800	13.3 X	78.6	28,393
Total			128	58,368	28.5 X	81.1	57,121^b

ISSN: 1471-2164