Table 7 Verification of FPC contig assembly by PCR amplicons designed hybridization markers.
From: Genomic tools development for Aquilegia: construction of a BAC-based physical map
Marker | Homology | Primer sequences | # positive hits (# contigsa) | # positive hits with expected amplicons |
|---|---|---|---|---|
Aq_SR_Ctg_2 | mlp-like protein 28 | Fwd AGGTGATGGAACCTGTGAGG Rev CACAATCCATGTCACCAAGC | 14 (3 +3sb) | 14 |
Aq_SR_Ctg_8 | No homology | Fwd GGCTATATCCACCAGGCTGA Rev AAGGGCCAGCACTTTATCCT | 12 (3) | 10 |
Aq_SR_Ctg_22 | Late embryogenesis-abundant protein | Fwd ATCATCCAACCTTGCGTTGT Rev GGGACCGGAACTATCCAAAT | 12 (2) | 12 |
Aq_SR_Ctg_30 | Ubiquitin-conjugating enzyme E2 | Fwd GCCCAAATCAAGAAACCAGA Rev CCTTTATGGACCCTGGATCA | 8 (1) | 8 |
Aq_SR_Ctg_118 | putative staygreen protein | Fwd TGGGGTCCACTTAAAGATGC Rev GAGTTGGTTGGTTTGGTTCC | 5 (3) | 5 |
Aq_SR_Ctg_127 | universal stress protein 1 | Fwd AGTAACTGGGCAAGCAGCAT Rev ATGGTGATGCAAGGGAAAAA | 6 (2) | 6 |
Aq_SR_Ctg_133 | ethylene-responsive transcriptional co-activator) | Fwd ATCGCATCGTCATCAAACAA Rev TTCAGCAGGCGTACGACGAG | 4 (2) | 3 |
Aq_SR_Ctg_144 | Benzodiazepine receptor-related | Fwd ACACTACGACATGCCAACCA Rev TAGCCCAGCCCAACAAATAG | 2 (1) | 2 |